ArticleNucleic acids research2011
Activation of a microRNA response in trans reveals a new role for poly(A) in translational repression.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Systematic Evaluation of Different Ribonucleoprotein Complexes as Posttranscriptional Biosensors in Cell-Free TX-TL Systems.ACS synthetic biology · 2026Article
- The Polypyrimidine Tract-Binding Protein Is a Transacting Factor for the Dengue Virus Internal Ribosome Entry Site.Viruses · 2024Article
- MicroRNA162 regulates stomatal conductance in response to low night temperature stressFrontiers in plant science · 2023Article
- The viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNA.PLoS pathogens · 2021Article
- Let-7a-regulated translational readthrough of mammalian AGO1 generates a microRNA pathway inhibitor.The EMBO journal · 2019Article
- Non-canonical translation initiation of the spliced mRNA encoding the human T-cell leukemia virus type 1 basic leucine zipper protein.Nucleic acids research · 2018Article
- microRNAs stimulate translation initiation mediated by HCV-like IRESes.Nucleic acids research · 2017Article
- MicroRNA-21 Lowers Blood Pressure in Spontaneous Hypertensive Rats by Upregulating Mitochondrial Translation.Circulation · 2016Article
- Article
- Improved dual AAV vectors with reduced expression of truncated proteins are safe and effective in the retina of a mouse model of Stargardt disease.Human molecular genetics · 2015Article
- The complexity of miRNA-mediated repression.Cell death and differentiation · 2015Review
- The panorama of miRNA-mediated mechanisms in mammalian cells.Cellular and molecular life sciences : CMLS · 2014Review
- A role for eIF4AII in microRNA-mediated mRNA silencing.Nature structural & molecular biology · 2013Article
- miRNA repression of translation in vitro takes place during 43S ribosomal scanning.Nucleic acids research · 2013Article
- Control of translation and miRNA-dependent repression by a novel poly(A) binding protein, hnRNP-Q.PLoS biology · 2013Article
- Alternative RISC assembly: binding and repression of microRNA-mRNA duplexes by human Ago proteins.RNA (New York, N.Y.) · 2012Article
- PABP and the poly(A) tail augment microRNA repression by facilitated miRISC binding.Nature structural & molecular biology · 2012Article
- Different effects of the TAR structure on HIV-1 and HIV-2 genomic RNA translation.Nucleic acids research · 2012Article
- A conserved PUF-Ago-eEF1A complex attenuates translation elongation.Nature structural & molecular biology · 2012Article
- A daphnane diterpenoid isolated from Wikstroemia polyantha induces an inflammatory response and modulates miRNA activity.PloS one · 2012Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we report that the untreated rabbit reticulocyte lysate contains over 300 different endogenous microRNAs together with the major components of the RNA-induced silencing complex and thus can be used as a model in vitro system to study the effects of microRNAs on gene expression. By using this system, we were able to show that microRNA hybridization to its target resulted in a very rapid and strong inhibition of expression that was exerted exclusively at the level of translation initiation with no involvement of transcript degradation or deadenylation. Moreover, we demonstrate that the magnitude of microRNA-induced repression can only be recapitulated in the context of a competitive translating environment. By using a wide spectrum of competitor cellular and viral RNAs, we could further show that competition was not exerted at the level of general components of the translational machinery, but relied exclusively on the presence of the poly(A) tail with virtually no involvement of the cap structure.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.