ArticleJournal of agricultural and food chemistry2011
N-linked glycan profiling of mature human milk by high-performance microfluidic chip liquid chromatography time-of-flight tandem mass spectrometry.
Article in Journal of agricultural and food chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01817127 (Functional Deconstruction of Human Milk Oligosaccharides and Lipids), which is not on this map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Functional Deconstruction of Human Milk Oligosaccharides and Lipids
Who cites it
19 citing papers in PubMed, 59 citations in OpenAlex.
- Identification and comparison ofFood chemistry: X · 2025Article
- Label-Free Liquid Chromatography-Mass Spectrometry Quantitation of RelativeInternational journal of molecular sciences · 2024Article
- Role of milk glycome in prevention, treatment, and recovery of COVID-19.Frontiers in nutrition · 2022Review
- Article
- Potential Applications of Endo-β-Frontiers in nutrition · 2021Review
- Serum Protein N-Glycans in Colostrum and Mature Milk of Chinese Mothers.Journal of agricultural and food chemistry · 2020Article
- Quantitative Longitudinal Inventory of theJournal of proteome research · 2020Article
- The Impact of Dietary Fucosylated Oligosaccharides and Glycoproteins of Human Milk on Infant Well-Being.Nutrients · 2020Review
- NIH workshop on human milk composition: summary and visions.The American journal of clinical nutrition · 2019Article
- Oligosaccharides Released from Milk Glycoproteins Are Selective Growth Substrates for Infant-Associated Bifidobacteria.Applied and environmental microbiology · 2016Article
- Following the digestion of milk proteins from mother to baby.Journal of proteome research · 2014Article
- Characterization of goat milk lactoferrin N-glycans and comparison with the N-glycomes of human and bovine milk.Electrophoresis · 2014Article
- Serum glycan signatures of gastric cancer.Cancer prevention research (Philadelphia, Pa.) · 2014Article
- Extensive in vivo human milk peptidomics reveals specific proteolysis yielding protective antimicrobial peptides.Journal of proteome research · 2013Article
- Consumption of human milk glycoconjugates by infant-associated bifidobacteria: mechanisms and implications.Microbiology (Reading, England) · 2013Review
- Analytical glycobiology at high sensitivity: current approaches and directions.Glycoconjugate journal · 2013Review
- Structural glycomic analyses at high sensitivity: a decade of progress.Annual review of analytical chemistry (Palo Alto, Calif.) · 2013Review
- Examination of glycan profiles from IgG-depleted human immunoglobulins facilitated by microscale affinity chromatography.Analytical chemistry · 2012Article
- Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
N-Linked glycans of skim human milk proteins were determined for three mothers. N-Linked glycans are linked to immune defense, cell growth, and cell-cell adhesion, but their functions in human milk are undetermined. Protein-bound N-linked glycans were released with peptidyl N-glycosidase F (PNGase F), enriched by graphitized carbon chromatography, and analyzed with Chip-TOF MS. To be defined as N-glycans, compounds were required, in all three procedural replicates, to match, within 6 ppm, against a theoretical human N-glycan library and be at least 2-fold higher in abundance in PNGase F-treated than in control samples. Fifty-two N-linked glycan compositions were identified, and 24 were confirmed via tandem mass spectra analysis. Twenty-seven compositions have been found previously in human milk, and 25 are novel compositions. By abundance, 84% of N-glycans were fucosylated and 47% were sialylated. The majority (70%) of total N-glycan abundance was composed of N-glycans found in all three milk samples.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.