ArticleEuropean journal of nutrition2011
Impact of spinach consumption on DNA stability in peripheral lymphocytes and on biochemical blood parameters: results of a human intervention trial.
Article in European journal of nutrition, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- DNA Damage Across Dietary Patterns: A Comet Assay Study in Vegans and Omnivores.Foods (Basel, Switzerland) · 2026Article
- Evaluation of Primary DNA Damage in Young Healthy Females Based on Their Dietary Preferences.Nutrients · 2023Article
- Influence of the Mediterranean Diet on Healthy Aging.International journal of molecular sciences · 2023Review
- Mediterranean diet and the hallmarks of ageing.European journal of clinical nutrition · 2021Review
- Spinach consumption and nonalcoholic fatty liver disease among adults: a case-control study.BMC gastroenterology · 2021Article
- Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study.Frontiers in nutrition · 2021Article
- Food intake biomarkers for green leafy vegetables, bulb vegetables, and stem vegetables: a review.Genes & nutrition · 2020Review
- Overview of Human Intervention Studies Evaluating the Impact of the Mediterranean Diet on Markers of DNA Damage.Nutrients · 2019Review
- Functional foods-based diet as a novel dietary approach for management of type 2 diabetes and its complications: A review.World journal of diabetes · 2014Review
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionA controlled intervention trial was conducted to assess the impact of spinach consumption on DNA stability in lymphocytes and on health-related biochemical parameters.
methodsThe participants (n = 8) consumed homogenised spinach (225 g/day/person) over a period of 16 days. DNA migration was monitored in single cell gel electrophoresis-comet assays under standard conditions, which reflect single- and double-strand breaks, after treatment of nuclei with lesion-specific enzymes (formamidopyrimidine glycosylase, FPG and endonuclease III, ENDO III) and after treatment of intact cells with H(2)O(2) before, during and after intervention.
resultsWhile no reduction in DNA damage was observed under standard conditions after different time intervals of spinach intake, other endpoints, namely ROS sensitivity and DNA migration attributable to the formation of oxidatively damaged DNA bases (i.e. pyrimidines-ENDO III-sensitive sites and purines-FPG sensitive sites) were reduced 6 h after consumption of the first portion and after 11 days of continuous consumption. In the case of ENDO III-sensitive sites, also after 16 days, a decrease in comet formation was observed. At the end of a 40 days washout period, the DNA stability parameters were not significantly different from the background values. Other biochemical parameters which were significantly altered by spinach intake were the folate (+27%) and homocysteine (-16%) concentrations in blood, and it was found in an earlier human study that folate may prevent oxidative damage to DNA bases.
conclusionsTaken together, our results show that moderate consumption of spinach causes protection against oxidative DNA damage in humans and that this phenomenon is paralleled by alterations of health-related biochemical parameters.
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