ArticlePloS one2011
In vitro dedifferentiation of melanocytes from adult epidermis.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Lentiviral hTERT Overexpression Extends the Lifespan of Primary Human Epidermal Melanocytes and Reshapes Transcriptional Programs.Biomolecules · 2026Article
- A Short Report on Melanocyte/Melanoma Culture, Senescence, and Reproducibility.Pigment cell & melanoma research · 2026Article
- Effect of Fibroblast Growth Factor-2 on Melanocyte Proliferation in Tissue-Engineered Skin Substitutes.International journal of molecular sciences · 2025Article
- Isolation and Culture of Epidermal Melanocytes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Isolation of tdTomato Expressing Inter-follicular Epidermal Melanocytes or Keratinocytes from Mouse Tail Skin.Bio-protocol · 2022Article
- BRAFeLife · 2021Article
- Dedifferentiation of human epidermal melanocytes in vitro by long-term trypsinization.Cell and tissue banking · 2021Article
- The tolerance of human epidermal cells to trypsinization in vitro.Cell and tissue banking · 2020Article
- Deciphering skin re-pigmentation patterns in vitiligo: an update on the cellular and molecular events involved.Chinese medical journal · 2020Review
- pH-controlled histone acetylation amplifies melanocyte differentiation downstream of MITF.EMBO reports · 2020Article
- Exploring Differential Connexin Expression across Melanocytic Tumor Progression Involving the Tumor Microenvironment.Cancers · 2019Article
- Quantitative analysis of melanin content in a three-dimensional melanoma cell culture.Scientific reports · 2019Article
- Tracking keratinocytes and melanocytes using carboxyfluorescein hydroxysuccinimidyl ester staining.PloS one · 2019Article
- Development and validation of a simple method for the extraction of human skin melanocytes.Cytotechnology · 2018Article
- Global Gene Expression Analysis in an in vitro Fibroblast Model of Idiopathic Pulmonary Fibrosis Reveals Potential Role for CXCL14/CXCR4.Scientific reports · 2018Article
- Differential miRNA expression profiles in human keratinocytes in response to protein kinase C inhibitor.Molecular medicine reports · 2017Article
- Melanosome transfer to keratinocyte in the chicken embryonic skin is mediated by vesicle release associated with Rho-regulated membrane blebbing.Scientific reports · 2016Article
- Self-Antigen Presentation by Keratinocytes in the Inflamed Adult Skin Modulates T-Cell Auto-Reactivity.The Journal of investigative dermatology · 2015Article
- Melanocytes--a novel tool to study mitochondrial dysfunction in Duchenne muscular dystrophy.Journal of cellular physiology · 2013Article
- New procedure for epidermal cell isolation using kiwi fruit actinidin, and improved culture of melanocytes in the presence of leukaemia inhibitory factor and forskolin.Cell proliferation · 2013Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In previous work we described a novel culture technique using a cholera toxin and PMA-free medium (Mel-mix) for obtaining pure melanocyte cultures from human adult epidermis. In Mel-mix medium the cultured melanocytes are bipolar, unpigmented and highly proliferative. Further characterization of the cultured melanocytes revealed the disappearance of c-Kit and TRP-1 and induction of nestin expression, indicating that melanocytes dedifferentiated in this in vitro culture. Cholera toxin and PMA were able to induce c-Kit and TRP-1 protein expressions in the cells, reversing dedifferentiation. TRP-1 mRNA expression was induced in dedifferentiated melanocytes by UV-B irradiated keratinocyte supernatants, however direct UV-B irradiation of the cells resulted in further decrease of TRP-1 mRNA expression. These dedifferentiated, easily accessible cultured melanocytes provide a good model for studying melanocyte differentiation and possibly transdifferentiation. Because melanocytes in Mel-mix medium can be cultured with human serum as the only supplement, this culture system is also suitable for autologous cell transplantation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.