ArticlePloS one2011
Identification of ER proteins involved in the functional organisation of the early secretory pathway in Drosophila cells by a targeted RNAi screen.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- p24-Tango1 interactions ensure ER-Golgi interface stability and efficient transport.The Journal of cell biology · 2024Article
- Engineering of TurboID-Wingless for the identification of Wingless interactors throughmicroPublication biology · 2024Article
- Article
- Tales of the ER-Golgi Frontier:Frontiers in cell and developmental biology · 2020Review
- Epistatic Analysis of the Contribution of Rabs and Kifs to CATCHR Family Dependent Golgi Organization.Frontiers in cell and developmental biology · 2019Article
- Pleiotropic effects of regulatory variation in tan result in correlation of two pigmentation traits in Drosophila melanogaster.Molecular ecology · 2018Article
- A Mechanism Coupling Systemic Energy Sensing to Adipokine Secretion.Developmental cell · 2017Article
- An Eye on Trafficking Genes: Identification of Four Eye Color Mutations in Drosophila.G3 (Bethesda, Md.) · 2016Article
- Screening for tumor suppressors: Loss of ephrin receptor A2 cooperates with oncogenic KRas in promoting lung adenocarcinoma.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The ROP vesicle release factor is required in adult Drosophila glia for normal circadian behavior.Frontiers in cellular neuroscience · 2015Article
- A stress assembly that confers cell viability by preserving ERES components during amino-acid starvation.eLife · 2014Article
- Retrograde traffic from the Golgi to the endoplasmic reticulum.Cold Spring Harbor perspectives in biology · 2013Review
- Substrate specificities and intracellular distributions of three N-glycan processing enzymes functioning at a key branch point in the insect N-glycosylation pathway.The Journal of biological chemistry · 2012Article
- Live-cell assays to identify regulators of ER-to-Golgi trafficking.Traffic (Copenhagen, Denmark) · 2012Article
- A genome-wide RNAi screen identifies regulators of cholesterol-modified hedgehog secretion in Drosophila.PloS one · 2012Article
- ERK7 is a negative regulator of protein secretion in response to amino-acid starvation by modulating Sec16 membrane association.The EMBO journal · 2011Article
- A haploid genetic screen identifies the major facilitator domain containing 2A (MFSD2A) transporter as a key mediator in the response to tunicamycin.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn Drosophila, the early secretory apparatus comprises discrete paired Golgi stacks in close proximity to exit sites from the endoplasmic reticulum (tER sites), thus forming tER-Golgi units. Although many components involved in secretion have been identified, the structural components sustaining its organisation are less known. Here we set out to identify novel ER resident proteins involved in the of tER-Golgi unit organisation.
resultsTo do so, we designed a novel screening strategy combining a bioinformatics pre-selection with an RNAi screen. We first selected 156 proteins exhibiting known or related ER retention/retrieval signals from a list of proteins predicted to have a signal sequence. We then performed a microscopy-based primary and confirmation RNAi screen in Drosophila S2 cells directly scoring the organisation of the tER-Golgi units. We identified 49 hits, most of which leading to an increased number of smaller tER-Golgi units (MG for "more and smaller Golgi") upon depletion. 16 of them were validated and characterised, showing that this phenotype was not due to an inhibition in secretion, a block in G2, or ER stress. Interestingly, the MG phenotype was often accompanied by an increase in the cell volume. Out of 6 proteins, 4 were localised to the ER.
conclusionsThis work has identified novel proteins involved in the organisation of the Drosophila early secretory pathway. It contributes to the effort of assigning protein functions to gene annotation in the secretory pathway, and analysis of the MG hits revealed an enrichment of ER proteins. These results suggest a link between ER localisation, aspects of cell metabolism and tER-Golgi structural organisation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.