ArticleThe Journal of biological chemistry2011
Essential role of protein phosphatase 2A in metaphase II arrest and activation of mouse eggs shown by okadaic acid, dominant negative protein phosphatase 2A, and FTY720.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Meiosis-specific distal cohesion site decoupled from the kinetochore.Nature communications · 2025Article
- Meiosis-specific decoupling of the pericentromere from the kinetochore.bioRxiv : the preprint server for biology · 2024Article
- Kinetochore individualization in meiosis I is required for centromeric cohesin removal in meiosis II.The EMBO journal · 2021Article
- Inflammatory cytokine-induced expression of MASTL is involved in hepatocarcinogenesis by regulating cell cycle progression.Oncology letters · 2019Article
- Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis.The Journal of cell biology · 2018Article
- Review
- PP2A regulates kinetochore-microtubule attachment during meiosis I in oocyte.Cell cycle (Georgetown, Tex.) · 2016Article
- MAPK3/1 (ERK1/2) and Myosin Light Chain Kinase in Mammalian Eggs Affect Myosin-II Function and Regulate the Metaphase II State in a Calcium- and Zinc-Dependent Manner.Biology of reproduction · 2015Article
- Physiologic Course of Female Reproductive Function: A Molecular Look into the Prologue of Life.Journal of pregnancy · 2015Review
- Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II.The Journal of cell biology · 2014Article
- The dynamics of MAPK inactivation at fertilization in mouse eggs.Journal of cell science · 2014Article
- α-endosulfine (ENSA) regulates exit from prophase I arrest in mouse oocytes.Cell cycle (Georgetown, Tex.) · 2014Article
- Release from meiotic arrest in ascidian eggs requires the activity of two phosphatases but not CaMKII.Development (Cambridge, England) · 2013Article
- Modulation of cell cycle control during oocyte-to-embryo transitions.The EMBO journal · 2013Review
- Sister chromatid segregation in meiosis II: deprotection through phosphorylation.Cell cycle (Georgetown, Tex.) · 2013Review
- Protein phosphatase 2A catalytic subunit α plays a MyD88-dependent, central role in the gene-specific regulation of endotoxin tolerance.Cell reports · 2013Article
- Expression of SET Protein in the Ovaries of Patients with Polycystic Ovary Syndrome.International journal of endocrinology · 2013Article
- DNA double strand breaks but not interstrand crosslinks prevent progress through meiosis in fully grown mouse oocytes.PloS one · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vertebrate eggs arrest at second meiotic metaphase. The fertilizing sperm causes meiotic exit through Ca(2+)-mediated activation of the anaphase-promoting complex/cyclosome (APC/C). Although the loss in activity of the M-phase kinase CDK1 is known to be an essential downstream event of this process, the contribution of phosphatases to arrest and meiotic resumption is less apparent, especially in mammals. Therefore, we explored the role of protein phosphatase 2A (PP2A) in mouse eggs using pharmacological inhibition and activation as well as a functionally dominant-negative catalytic PP2A subunit (dn-PP2Ac-L199P) coupled with live cell imaging. We observed that PP2A inhibition using okadaic acid induced events normally observed at fertilization: degradation of the APC/C substrates cyclin B1 and securin resulting from loss of the APC/C inhibitor Emi2. Although sister chromatids separated, chromatin remained condensed, and polar body extrusion was blocked as a result of a rapid spindle disruption, which could be ameliorated by non-degradable cyclin B1, suggesting that spindle integrity was affected by CDK1 loss. Similar cell cycle effects to okadaic acid were also observed using dominant-negative PP2Ac. Preincubation of eggs with the PP2A activator FTY720 could block many of the actions of okadaic acid, including Emi2, cyclin B1, and securin degradation and sister chromatid separation. Therefore, in conclusion, we used okadaic acid, dn-PP2Ac-L199P, and FTY720 on mouse eggs to demonstrate that PP2A is needed to for both continued metaphase arrest and successful exit from meiosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.