ArticleThe Journal of biological chemistry2011
The adipophilin C terminus is a self-folding membrane-binding domain that is important for milk lipid secretion.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
- PLIN2-PGAM5-regulated lipid droplet-mitochondria contacts drive microglial neuroinflammation after spinal cord injury via fatty acid metabolic reprogramming.Cell death and differentiation · 2026Article
- Perilipin-2 promotes lipid droplet-plasma membrane interactions that facilitate apocrine lipid secretion in secretory epithelial cells of the mouse mammary gland.Frontiers in cell and developmental biology · 2022Article
- Occludin is a target of Src kinase and promotes lipid secretion by binding to BTN1a1 and XOR.PLoS biology · 2022Article
- Validating an artificial organelle: Studies of lipid droplet-specific proteins on adiposome platform.iScience · 2021Article
- Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish.eLife · 2021Article
- ADFP promotes cell proliferation in lung adenocarcinoma via Akt phosphorylation.Journal of cellular and molecular medicine · 2021Article
- Dual binding motifs underpin the hierarchical association of perilipins1-3 with lipid droplets.Molecular biology of the cell · 2019Article
- Compositional Dynamics of the Milk Fat Globule and Its Role in Infant Development.Frontiers in pediatrics · 2018Review
- The endoplasmic reticulum and casein-containing vesicles contribute to milk fat globule membrane.Molecular biology of the cell · 2016Article
- Targeting Fat: Mechanisms of Protein Localization to Lipid Droplets.Trends in cell biology · 2016Review
- Conserved Amphipathic Helices Mediate Lipid Droplet Targeting of Perilipins 1-3.The Journal of biological chemistry · 2016Article
- Structural and functional assessment of perilipin 2 lipid binding domain(s).Biochemistry · 2014Article
- Development of high-sensitive ELISA method for detection of adipophilin levels in human colostrum and breast milk.Journal of clinical laboratory analysis · 2014Article
- Article
- Sequencing the transcriptome of milk production: milk trumps mammary tissue.BMC genomics · 2013Article
- A test of current models for the mechanism of milk-lipid droplet secretion.Traffic (Copenhagen, Denmark) · 2013Article
- Article
- Article
- Milk lipid secretion: recent biomolecular aspects.Biomolecular concepts · 2012Article
- Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Cytoplasmic lipid droplets (CLD) in mammary epithelial cells undergo secretion by a unique membrane envelopment process to produce milk lipids. Adipophilin (ADPH/Plin2), a member of the perilipin/PAT family of lipid droplet-associated proteins, is hypothesized to mediate CLD secretion through interactions with apical plasma membrane elements. We found that the secretion of CLD coated by truncated ADPH lacking the C-terminal region encoding a putative four-helix bundle structure was impaired relative to that of CLD coated by full-length ADPH. We used homology modeling and analyses of the solution and membrane binding properties of purified recombinant ADPH C terminus to understand how this region possibly mediates CLD secretion. Homology modeling supports the concept that the ADPH C terminus forms a four-helix bundle motif and suggests that this structure can form stable membrane bilayer interactions. Circular dichroism and protease mapping studies confirmed that the ADPH C terminus is an independently folding α-helical structure that is relatively resistant to urea denaturation. Liposome binding studies showed that the purified C terminus binds to phospholipid membranes through electrostatic dependent interactions, and cell culture studies documented that it localizes to the plasma membrane. Collectively, these data provide direct evidence that the ADPH C terminus forms a stable membrane binding helical structure that is important for CLD secretion. We speculate that interactions between the four-helix bundle of ADPH and membrane phospholipids may be an initial step in milk lipid secretion.
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