ArticleJournal of lipid research2011
Quantification of sterol lipids in plants by quadrupole time-of-flight mass spectrometry.
Article in Journal of lipid research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed.
- Review
- Diagnostic Fragmentations of Animal and Fungal Sterols/Stanols Obtained by APCI-Tandem Mass Spectrometry: A Route Towards Unknown Free Sterol Identification.Metabolites · 2025Article
- In vitro effects of potato glycoalkaloids on plant-pathogens, beneficial microbes, and Arabidopsis thaliana.Scientific reports · 2025Article
- Sterols in plant biologyCell surface (Amsterdam, Netherlands) · 2025Review
- Specific Transcriptional Regulation Controls Plant Organ-Specific Infection by the Oomycete Pathogen Phytophthora sojae.Molecular plant pathology · 2024Article
- Exploring the plant lipidome: techniques, challenges, and prospects.Advanced biotechnology · 2024Review
- Advances and Challenges in Plant Sterol Research: Fundamentals, Analysis, Applications and Production.Molecules (Basel, Switzerland) · 2023Review
- Optimization of Magnetic and Paper-Based Molecularly Imprinted Polymers for Selective Extraction of Charantin inInternational journal of molecular sciences · 2023Article
- Nitrated Fatty-Acids Distribution in Storage Biomolecules duringAntioxidants (Basel, Switzerland) · 2022Article
- Activation of neutral sphingomyelinase 2 through hyperglycemia contributes to endothelial apoptosis via vesicle-bound intercellular transfer of ceramides.Cellular and molecular life sciences : CMLS · 2021Article
- An Arabidopsis lipid map reveals differences between tissues and dynamic changes throughout development.The Plant journal : for cell and molecular biology · 2021Article
- Chemical profiling of root bark extract fromPeerJ · 2021Article
- Targeted Analysis of the Plant Lipidome by UPLC-NanoESI-MS/MS.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Glycerolipid profile differences between perennial and annual stem zones in the perennial model plantPlant direct · 2021Article
- Development of Methods to Quantify Free and Conjugated Steroids in Fatty Matrices by HPLC-MS/MS.ACS omega · 2020Article
- Responses of Arbuscular Mycorrhizal Symbiosis to Abiotic Stress: A Lipid-Centric Perspective.Frontiers in plant science · 2020Review
- Ultrahigh-pressure supercritical fluid extraction and chromatography of Moringa oleifera and Moringa peregrina seed lipids.Analytical and bioanalytical chemistry · 2019Article
- Involvement of Phosphatidylserine and Triacylglycerol in the Response of Sweet Potato Leaves to Salt Stress.Frontiers in plant science · 2019Article
- Brassinosteroids Affect the Symbiosis Between the AM FungusFrontiers in plant science · 2019Article
- Terpenoid Esters Are the Major Constituents From Leaf Lipid Droplets ofFrontiers in plant science · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycerolipids, sphingolipids, and sterol lipids constitute the major lipid classes in plants. Sterol lipids are composed of free and conjugated sterols, i.e., sterol esters, sterol glycosides, and acylated sterol glycosides. Sterol lipids play crucial roles during adaption to abiotic stresses and plant-pathogen interactions. Presently, no comprehensive method for sterol lipid quantification in plants is available. We used nanospray ionization quadrupole-time-of-flight mass spectrometry (Q-TOF MS) to resolve and identify the molecular species of all four sterol lipid classes from Arabidopsis thaliana. Free sterols were derivatized with chlorobetainyl chloride. Sterol esters, sterol glycosides, and acylated sterol glycosides were ionized as ammonium adducts. Quantification of molecular species was achieved in the positive mode after fragmentation in the presence of internal standards. The amounts of sterol lipids quantified by Q-TOF MS/MS were validated by comparison with results obtained with TLC/GC. Quantification of sterol lipids from leaves and roots of phosphate-deprived A. thaliana plants revealed changes in the amounts and molecular species composition. The Q-TOF method is far more sensitive than GC or HPLC. Therefore, Q-TOF MS/MS provides a comprehensive strategy for sterol lipid quantification that can be adapted to other tandem mass spectrometers.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.