ArticlePloS one2011
Zebrafish bioassay-guided natural product discovery: isolation of angiogenesis inhibitors from East African medicinal plants.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 87 citations in OpenAlex.
- Article
- Gut microbiota metabolically mediate intestinal helminth infection in Zebrafish.bioRxiv : the preprint server for biology · 2024Article
- Danthron, an Anthraquinone Isolated from a Marine Fungus, Is a New Inhibitor of Angiogenesis Exhibiting Interesting Antitumor and Antioxidant Properties.Antioxidants (Basel, Switzerland) · 2023Article
- Investigation of Kelussia Odoratissima and Angelica Sinensis Similarities in Zebrafish-based In-vivo Bioactivity Assays and Their Chemical Composition.Galen medical journal · 2023Article
- Effects of carnosine on the embryonic development and TiOFrontiers in veterinary science · 2023Article
- Acute Toxicity and Anti-Inflammatory Activity ofMolecules (Basel, Switzerland) · 2022Article
- Australindolones, New Aminopyrimidine Substituted Indolone Alkaloids from an Antarctic TunicateMarine drugs · 2022Article
- In vivo Screening of Natural Products Against Angiogenesis and Mechanisms of Anti-Angiogenic Activity of Deoxysappanone B 7,4'-Dimethyl Ether.Drug design, development and therapy · 2020Article
- Zebrafish as an Emerging Model for Bioassay-Guided Natural Product Drug Discovery for Neurological Disorders.Medicines (Basel, Switzerland) · 2019Review
- Comparison the Effect of Ferutinin and 17β-Estradiol on Bone Mineralization of Developing Zebrafish (International journal of molecular sciences · 2019Article
- Protein Kinase CK2, a Potential Therapeutic Target in Carcinoma ManagementAsian Pacific journal of cancer prevention : APJCP · 2019Review
- Review
- Kaempferol Identified by Zebrafish Assay and Fine Fractionations Strategy from Dysosma versipellis Inhibits Angiogenesis through VEGF and FGF Pathways.Scientific reports · 2015Article
- Phenotype-Guided Natural Products Discovery Using Cytological Profiling.Journal of natural products · 2015Article
- Short-term exposure to low doses of rotenone induces developmental, biochemical, behavioral, and histological changes in fish.Environmental science and pollution research international · 2015Article
- Connecting phenotype and chemotype: high-content discovery strategies for natural products research.Journal of natural products · 2015Review
- Fishing on chips: up-and-coming technological advances in analysis of zebrafish and Xenopus embryos.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2014Review
- Hooking the big one: the potential of zebrafish xenotransplantation to reform cancer drug screening in the genomic era.Disease models & mechanisms · 2014Review
- Matching the power of high throughput screening to the chemical diversity of natural products.Natural product reports · 2013Review
- Functional inhibition of UQCRB suppresses angiogenesis in zebrafish.Biochemical and biophysical research communications · 2013Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Natural products represent a significant reservoir of unexplored chemical diversity for early-stage drug discovery. The identification of lead compounds of natural origin would benefit from therapeutically relevant bioassays capable of facilitating the isolation of bioactive molecules from multi-constituent extracts. Towards this end, we developed an in vivo bioassay-guided isolation approach for natural product discovery that combines bioactivity screening in zebrafish embryos with rapid fractionation by analytical thin-layer chromatography (TLC) and initial structural elucidation by high-resolution electrospray mass spectrometry (HRESIMS). Bioactivity screening of East African medicinal plant extracts using fli-1:EGFP transgenic zebrafish embryos identified Oxygonum sinuatum and Plectranthus barbatus as inhibiting vascular development. Zebrafish bioassay-guided fractionation identified the active components of these plants as emodin, an inhibitor of the protein kinase CK2, and coleon A lactone, a rare abietane diterpenoid with no previously described bioactivity. Both emodin and coleon A lactone inhibited mammalian endothelial cell proliferation, migration, and tube formation in vitro, as well as angiogenesis in the chick chorioallantoic membrane (CAM) assay. These results suggest that the combination of zebrafish bioassays with analytical chromatography methods is an effective strategy for the rapid identification of bioactive natural products.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.