ArticlePloS one2011
Differential proteomic analysis of platelets suggested possible signal cascades network in platelets treated with salvianolic acid B.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Review
- Preparation and sustained-release properties of poly(lactic acid)/graphene oxide porous biomimetic composite scaffolds loaded with salvianolic acid B.RSC advances · 2022Article
- Review
- Mechanism and Potential Target of Blood-Activating Chinese Botanical Drugs Combined With Anti-Platelet Drugs: Prevention and Treatment of Atherosclerotic Cardiovascular Diseases.Frontiers in pharmacology · 2022Review
- The CPNE Family and Their Role in Cancers.Frontiers in genetics · 2021Review
- Tannins as Hemostasis Modulators.Frontiers in pharmacology · 2021Review
- The Effect of Salvianolic Acid on Vascular Protection and Possible Mechanisms.Oxidative medicine and cellular longevity · 2020Review
- Pharmacological Effects of Salvianolic Acid B Against Oxidative Damage.Frontiers in pharmacology · 2020Review
- Differential proteomics for studying action mechanisms of traditional Chinese medicines.Chinese medicine · 2019Review
- Salvia miltiorrhizaBurge (Danshen): a golden herbal medicine in cardiovascular therapeutics.Acta pharmacologica Sinica · 2018Review
- Application of Feedback System Control Optimization Technique in Combined Use of Dual Antiplatelet Therapy and Herbal Medicines.Frontiers in physiology · 2018Article
- Differential proteomic analysis of platelets suggested target-related proteins in rabbit platelets treated with Rhizoma Corydalis.Pharmaceutical biology · 2017Article
- The Traditional Medicine and Modern Medicine from Natural Products.Molecules (Basel, Switzerland) · 2016Review
- Heat shock protein 70 regulates platelet integrin activation, granule secretion and aggregation.American journal of physiology. Cell physiology · 2016Article
- Chemical Analysis of the Herbal Medicine Salviae miltiorrhizae Radix et Rhizoma (Danshen).Molecules (Basel, Switzerland) · 2016Review
- Recent Advance in Applications of Proteomics Technologies on Traditional Chinese Medicine Research.Evidence-based complementary and alternative medicine : eCAM · 2015Review
- Natural Products for Antithrombosis.Evidence-based complementary and alternative medicine : eCAM · 2015Review
- Pretreatment with Danhong injection protects the brain against ischemia-reperfusion injury.Neural regeneration research · 2014Article
- Cardiovascular effects of salvianolic Acid B.Evidence-based complementary and alternative medicine : eCAM · 2013Article
- Network pharmacology: a new approach for chinese herbal medicine research.Evidence-based complementary and alternative medicine : eCAM · 2013Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSalvianolic acid B (SB) is an active component isolated from Danshen, a traditional Chinese medicine widely used for the treatment of cardiovascular disorders. Previous study suggested that SB might inhibit adhesion as well as aggregation of platelets by a mechanism involving the integrin α2β1. But, the signal cascades in platelets after SB binding are still not clear. METHODOLOGY/PRINCIPAL
findingsIn the present study, a differential proteomic analysis (two-dimensional electrophoresis) was conducted to check the protein expression profiles of rat platelets with or without treatment of SB. Proteins altered in level after SB exposure were identified by MALDI-TOF MS/MS. Treatment of SB caused regulation of 20 proteins such as heat shock-related 70 kDa protein 2 (hsp70), LIM domain protein CLP-36, copine I, peroxiredoxin-2, coronin-1 B and cytoplasmic dynein intermediate chain 2C. The regulation of SB on protein levels was confirmed by Western blotting. The signal cascades network induced by SB after its binding with integrin α2β1 was predicted. To certify the predicted network, binding affinity of SB to integrin α2β1 was checked in vitro and ex vivo in platelets. Furthermore, the effects of SB on protein levels of hsp70, coronin-1B and intracellular levels of Ca²+ and reactive oxygen species (ROS) were checked with or without pre-treatment of platelets using antibody against integrin α2β1. Electron microscopy study confirmed that SB affected cytoskeleton structure of platelets. CONCLUSIONS/SIGNIFICANCE: Integrin α2β1 might be one of the direct target proteins of SB in platelets. The signal cascades network of SB after binding with integrin α2β1 might include regulation of intracellular Ca²+ level, cytoskeleton-related proteins such as coronin-1B and cytoskeleton structure of platelets.
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