ArticleNature methods2011
Mapping of signaling networks through synthetic genetic interaction analysis by RNAi.
Article in Nature methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
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Who cites it
110 citing papers in PubMed.
- Article
- Behavioral screening defines the molecular Parkinsonism-related subgroups in Drosophila.Nature communications · 2026Article
- Quantitative analysis of genetic interactions in human cells from genome-wide CRISPR-Cas9 screens.bioRxiv : the preprint server for biology · 2026Article
- Synthetic lethality in cancer drug discovery: challenges and opportunities.Nature reviews. Drug discovery · 2026Review
- A mathematical framework for the quantitative analysis of genetic buffering.PLoS genetics · 2025Article
- A multilineage screen identifies actionable synthetic lethal interactions in human cancers.Nature genetics · 2025Article
- Review
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- Leveraging the genetic correlation between traits improves the detection of epistasis in genome-wide association studies.G3 (Bethesda, Md.) · 2023Article
- A global genetic interaction network by single-cell imaging and machine learning.Cell systems · 2023Article
- From systems to structure - using genetic data to model protein structures.Nature reviews. Genetics · 2022Review
- Genetic landscape of T cells identifies synthetic lethality for T-ALL.Communications biology · 2021Article
- Functional assessment of the "two-hit" model for neurodevelopmental defects in Drosophila and X. laevis.PLoS genetics · 2021Article
- Revealing biomarkers associated with PARP inhibitors based on genetic interactions in cancer genome.Computational and structural biotechnology journal · 2021Article
- Targeted Perturb-seq enables genome-scale genetic screens in single cells.Nature methods · 2020Article
- Article
- Article
- A Gaussian process-based definition reveals new and bona fide genetic interactions compared to a multiplicative model in the Gram-negative Escherichia coli.Bioinformatics (Oxford, England) · 2020Article
- Article
- A one-step tRNA-CRISPR system for genome-wide genetic interaction mapping in mammalian cells.Scientific reports · 2019Article
50 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The analysis of synthetic genetic interaction networks can reveal how biological systems achieve a high level of complexity with a limited repertoire of components. Studies in yeast and bacteria have taken advantage of collections of deletion strains to construct matrices of quantitative interaction profiles and infer gene function. Yet comparable approaches in higher organisms have been difficult to implement in a robust manner. Here we report a method to identify genetic interactions in tissue culture cells through RNAi. By performing more than 70,000 pairwise perturbations of signaling factors, we identified >600 interactions affecting different quantitative phenotypes of Drosophila melanogaster cells. Computational analysis of this interaction matrix allowed us to reconstruct signaling pathways and identify a conserved regulator of Ras-MAPK signaling. Large-scale genetic interaction mapping by RNAi is a versatile, scalable approach for revealing gene function and the connectivity of cellular networks.
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