ArticleNature methods2011
Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination.
Article in Nature methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 389 papers.
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Who cites it
389 citing papers in PubMed.
- Two-photon light-sheet microscopy with isotropic near-diffraction-limited resolution in three dimensions.iScience · 2026Article
- Space-time light-sheet microscopy.Nature communications · 2026Article
- Scanning Bessel beam microscopy with a protected and corrective objective for solvent-cleared large samples.iScience · 2026Article
- Two-photon 3D imaging of optically stimulated neural activity at 100 Hz.Light, science & applications · 2026Article
- Tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric imaging of brain dynamics.eLife · 2026Article
- A multimodal adaptive optical microscope for in vivo imaging from molecules to organisms.Nature methods · 2026Article
- Polarization-engineered aberration-resilient light sheet microscopy.bioRxiv : the preprint server for biology · 2026Article
- pan-ASLM: Axially Swept Light Sheet Microscopy for Fast and High-Resolution Imaging of Expanded Samples.Npj imaging · 2026Article
- Article
- Hyperspectral coherent anti-Stokes Raman scattering volumetric projection microscopy for assessing drug-cell interaction.Biomedical optics express · 2026Article
- Single-Objective Lattice Light Sheet Microscopy with Microfluidics for Single-Molecule Super-Resolution Imaging of Mammalian Cells.ACS photonics · 2026Article
- Tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric imaging of brain dynamics.bioRxiv : the preprint server for biology · 2026Article
- Emerging Light-Sheet Fluorescence Microscopy for Cardiac Vascular Imaging in Zebrafish.Current treatment options in cardiovascular medicine · 2026Review
- Altair-LSFM A High-Resolution, Easy-to-Build Light-Sheet Microscope for Sub-Cellular Imaging.bioRxiv : the preprint server for biology · 2025Article
- Repeated extrinsic and anisotropic mechanical inputs promote C. elegans polarized adherens junction elongation.Developmental cell · 2025Article
- Axicons for improved resolution and depth of focus in adaptive optics scanning light ophthalmoscopy.Biomedical optics express · 2025Article
- Advancing Multicolor Super-Resolution Volume Imaging: Illuminating Complex Cellular Dynamics.JACS Au · 2025Review
- A Multimodal Adaptive Optical Microscope ForbioRxiv : the preprint server for biology · 2025Article
- Radial rotation of cell-pair under beam mode coupling effect of microcavity cascaded single fiber optical tweezers.Nanophotonics (Berlin, Germany) · 2025Article
- Deep learning enhanced light sheet fluorescence microscopy for in vivo 4D imaging of zebrafish heart beating.Light, science & applications · 2025Article
329 more citing papers are in PubMed but not listed here.
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7 authors.
Funding
Abstract
A key challenge when imaging living cells is how to noninvasively extract the most spatiotemporal information possible. Unlike popular wide-field and confocal methods, plane-illumination microscopy limits excitation to the information-rich vicinity of the focal plane, providing effective optical sectioning and high speed while minimizing out-of-focus background and premature photobleaching. Here we used scanned Bessel beams in conjunction with structured illumination and/or two-photon excitation to create thinner light sheets (<0.5 μm) better suited to three-dimensional (3D) subcellular imaging. As demonstrated by imaging the dynamics of mitochondria, filopodia, membrane ruffles, intracellular vesicles and mitotic chromosomes in live cells, the microscope currently offers 3D isotropic resolution down to ∼0.3 μm, speeds up to nearly 200 image planes per second and the ability to noninvasively acquire hundreds of 3D data volumes from single living cells encompassing tens of thousands of image frames.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.