ArticleNature biotechnology2011
Conversion of proteins into biofuels by engineering nitrogen flux.
Article in Nature biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
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Who cites it
68 citing papers in PubMed.
- Enhanced growth of 1-butanol producingBiodesign research · 2026Article
- Integrating synthetic biology and process engineering for enhanced isobutanol biosynthesis yield and sustainability.Biotechnology for biofuels and bioproducts · 2026Review
- Adaptive Laboratory Evolution and Carbon/Nitrogen Imbalance Promote High-Yield Ammonia Release inMicroorganisms · 2025Article
- Recent advances in microbial 3-methyl-1-butanol production.Frontiers in microbiology · 2025Review
- Engineering thioesterase as a driving force for novel itaconate production via its degradation scheme.Metabolic engineering communications · 2024Article
- Concentration Recognition-Based Auto-Dynamic Regulation System (CRUISE) Enabling Efficient Production of Higher Alcohols.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Review
- Biosynthesis pathways of expanding carbon chains for producing advanced biofuels.Biotechnology for biofuels and bioproducts · 2023Review
- Functional Carbon from Nature: Biomass-Derived Carbon Materials and the Recent Progress of Their Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Ammonia Production Using Bacteria and Yeast toward a Sustainable Society.Bioengineering (Basel, Switzerland) · 2023Review
- Enhanced isobutanol production using engineered3 Biotech · 2022Article
- Genetic and metabolic engineering for poly-γ-glutamic acid production: current progress, challenges, and prospects.World journal of microbiology & biotechnology · 2022Review
- Toward bioproduction of oxo chemicals from CBiotechnology for biofuels and bioproducts · 2022Review
- Harnessing plasmid replication mechanism to enable dynamic control of gene copy in bacteria.Metabolic engineering · 2022Article
- Autophagy Improves ARA-Rich TAG Accumulation inMicrobiology spectrum · 2022Article
- Techno-economic assessment for the production of algal fuels and value-added products: opportunities for high-protein microalgae conversion.Biotechnology for biofuels and bioproducts · 2022Article
- Metabolic Engineering for Valorization of Agri- and Aqua-Culture Sidestreams for Production of Nitrogenous Compounds byFrontiers in microbiology · 2022Review
- Microbial synthesis of 4-hydroxybenzoic acid from renewable feedstocks.Food chemistry. Molecular sciences · 2021Article
- Metabolic engineering of Escherichia coli for the production of isobutanol: a review.World journal of microbiology & biotechnology · 2021Review
- Exploring the Tunability and Dynamic Properties of MarR-PmarO Sensor System inACS synthetic biology · 2021Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofuels are currently produced from carbohydrates and lipids in feedstock. Proteins, in contrast, have not been used to synthesize fuels because of the difficulties of deaminating protein hydrolysates. Here we apply metabolic engineering to generate Escherichia coli that can deaminate protein hydrolysates, enabling the cells to convert proteins to C4 and C5 alcohols at 56% of the theoretical yield. We accomplish this by introducing three exogenous transamination and deamination cycles, which provide an irreversible metabolic force that drives deamination reactions to completion. We show that Saccharomyces cerevisiae, E. coli, Bacillus subtilis and microalgae can be used as protein sources, producing up to 4,035 mg/l of alcohols from biomass containing ∼22 g/l of amino acids. These results show the feasibility of using proteins for biorefineries, for which high-protein microalgae could be used as a feedstock with a possibility of maximizing algal growth and total CO(2) fixation.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.