ArticleThe Journal of experimental medicine1990
Intracellular application of guanosine-5'-O-(3-thiotriphosphate) induces exocytotic granule fusion in guinea pig eosinophils.
Article in The Journal of experimental medicine, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 66 citations in OpenAlex.
- Eosinophil biology in COPD: from scientific insight to clinical treatment action.ERJ open research · 2026Review
- Mechanisms of Eosinophil Degranulation.Cells · 2026Review
- Eosinophil cytokines, chemokines, and growth factors: emerging roles in immunity.Frontiers in immunology · 2014Review
- CCL11 elicits secretion of RNases from mouse eosinophils and their cell-free granules.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Eosinophil function in allergic inflammation: from bone marrow to tissue response.Current allergy and asthma reports · 2004Review
- Mechanisms of eosinophil recruitment and activation.Current allergy and asthma reports · 2002Review
- Fusion pore expansion in horse eosinophils is modulated by Ca2+ and protein kinase C via distinct mechanisms.The EMBO journal · 1998Article
- Induction of exocytosis from permeabilized mast cells by the guanosine triphosphatases Rac and Cdc42.Molecular biology of the cell · 1998Article
- A novel thromboxane synthetase inhibitor, DP-1904, inhibits human blood eosinophil degranulation.Inflammation · 1997Article
- Rho guanine nucleotide dissociation inhibitor protein (RhoGDI) inhibits exocytosis in mast cells.The EMBO journal · 1996Article
- Purification and identification of FOAD-II, a cytosolic protein that regulates secretion in streptolysin-O permeabilized mast cells, as a rac/rhoGDI complex.Molecular biology of the cell · 1996Article
- Differential regulation of von Willebrand factor exocytosis and prostacyclin synthesis in electropermeabilized endothelial cell monolayers.The Biochemical journal · 1995Article
- Regulation of granule size in human and horse eosinophils by number of fusion events among unit granules.The Journal of physiology · 1995Article
- Eosinophils in the 1990s: new perspectives on their role in health and disease.Postgraduate medical journal · 1994Review
- Focal exocytosis by eosinophils--compound exocytosis and cumulative fusion.The EMBO journal · 1993Article
- The exocytotic fusion pore.The Journal of cell biology · 1992Review
- Guanine nucleotide is essential and Ca2+ is a modulator in the exocytotic reaction of permeabilized rat mast cells.The Biochemical journal · 1992Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The mechanism of eosinophil secretion was studied in guinea pig eosinophils by measuring release of hexosaminidase from cell suspensions (greater than 98% pure) permeabilized with streptolysin-O and by whole-cell patch-clamp capacitance measurements. It is shown that release of eosinophil granule components occurs by an exocytotic mechanism in which individual granules fuse with the plasma membrane. Exocytosis can be induced by intracellular application of the nonhydrolyzable GTP analog guanosine-5'-O-(3-thiotriphosphate) (GTP-gamma-S), suggesting the involvement of a GTP-binding protein. The activation is modulated by the intracellular calcium concentration, with activation by GTP-gamma-S inducing transient elevations in the concentration of Ca2+. Thus, the nature and regulation of the release mechanism appear to be very similar to that of the mast cell and neutrophil.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.