ArticleCardiovascular research2011
Enhanced contractility and myosin phosphorylation induced by Ca(2+)-independent MLCK activity in hypertensive rats.
Article in Cardiovascular research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Regulation of Vascular Tone of Preglomerular Renal Vasculature by Caldesmon.Journal of the American Heart Association · 2026Article
- Ventral stress fibers induce plasma membrane deformation in human fibroblasts.Molecular biology of the cell · 2021Article
- Cadmium-induced hypertension is associated with renal myosin light chain phosphatase inhibition via increased T697 phosphorylation and p44 mitogen-activated protein kinase levels.Hypertension research : official journal of the Japanese Society of Hypertension · 2021Article
- LRP1 (Low-Density Lipoprotein Receptor-Related Protein 1) Regulates Smooth Muscle Contractility by Modulating CaArteriosclerosis, thrombosis, and vascular biology · 2018Article
- Novel Functions of Death-Associated Protein Kinases through Mitogen-Activated Protein Kinase-Related Signals.International journal of molecular sciences · 2018Review
- Involvement of inhibitor kappa B kinase 2 (IKK2) in the regulation of vascular tone.Laboratory investigation; a journal of technical methods and pathology · 2018Article
- Characterization of pressure-mediated vascular tone in resistance arteries from bile duct-ligated rats.Oncotarget · 2017Article
- Myosin Light Chain Kinase: A Potential Target for Treatment of Inflammatory Diseases.Frontiers in pharmacology · 2017Review
- Inhibition of endoplasmic reticulum stress improves coronary artery function in the spontaneously hypertensive rats.Scientific reports · 2016Article
- Obesity, Inflammation, and Exercise Training: Relative Contribution of iNOS and eNOS in the Modulation of Vascular Function in the Mouse Aorta.Frontiers in physiology · 2016Article
- Highly sensitive myosin phosphorylation analysis in the renal afferent arteriole.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2016Review
- In vivo roles for myosin phosphatase targeting subunit-1 phosphorylation sites T694 and T852 in bladder smooth muscle contraction.The Journal of physiology · 2015Article
- Low K⁺ current in arterial myocytes with impaired K⁺-vasodilation and its recovery by exercise in hypertensive rats.Pflugers Archiv : European journal of physiology · 2014Article
- Involvement of myosin regulatory light chain diphosphorylation in sustained vasoconstriction under pathophysiological conditions.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2014Review
- Role of endogenous ENaC and TRP channels in the myogenic response of rat posterior cerebral arteries.PloS one · 2013Article
- New insights into myosin phosphorylation during cyclic nucleotide-mediated smooth muscle relaxation.Journal of muscle research and cell motility · 2012Article
- Myosin regulatory light chain diphosphorylation slows relaxation of arterial smooth muscle.The Journal of biological chemistry · 2012Article
- The role of sphingosine kinase 1/sphingosine-1-phosphate pathway in the myogenic tone of posterior cerebral arteries.PloS one · 2012Article
- Role for PKCβ in enhanced endothelin-1-induced pulmonary vasoconstrictor reactivity following intermittent hypoxia.American journal of physiology. Lung cellular and molecular physiology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
aimsThe role of Ca(2+) sensitization induced by a Ca(2+)-independent myosin light chain kinase (MLCK) in hypertension has not been determined. The aim of this study was to clarify the role of possible Ca(2+)-independent MLCK activity in hypertension. METHODS AND
resultsWe compared increases in contractile force and phosphorylation of myosin light chain (MLC) evoked by calyculin A, a phosphatase inhibitor, in β-escin-permeabilized mesenteric arteries at pCa 9.0 between spontaneously hypertensive rat (SHR) and Wistar Kyoto rat (WKY). We found that there was no detectable phosphorylation of MLC at pCa 9.0, but that the administration of 1 μM calyculin A gradually increased force and mono- and di-phosphorylation of MLC. This contraction was inhibited by staurosporine but not by wortmannin, Y-27632, or calphostin-C. The calyculin A-induced contraction was significantly greater in the SHR than in the WKY and was associated with an increase in mono- and di-phosphorylation of MLC. SM-1, a zipper-interacting protein kinase (ZIPK)-inhibiting peptide, significantly inhibited the amplitude of the calyculin A-induced contraction and di-phosphorylation. Total ZIPK expression (54 + 32 kDa) was greater in the SHR than in the WKY. Phosphorylation of myosin phosphatase target subunit at Thr(697), but not at Thr(855), was consistently stronger in the SHR than in the WKY in calyculin A-treated tissues at pCa 9.0.
conclusionsOur results suggest that Ca(2+)-independent MLCK activity is enhanced in the SHR, and that ZIPK plays, at least in part, an important role as a candidate for this kinase in rat mesenteric arteries.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.