ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2011
A novel histochemical method for a simultaneous staining of melanin and collagen fibers.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Comparison of laboratory and clinical parameters of cold versus warm trypsinization methods of non-cultured epidermal cell suspension preparation in the treatment of stable vitiligo: a randomized trial.Archives of dermatological research · 2024Trial
- Article
- A comprehensive analysis of two types of xenogeneic bone particles for use in maxillofacial bone regeneration therapies.PloS one · 2025Article
- Melanometry for objective evaluation of skin pigmentation in pulse oximetry studies.Communications medicine · 2024Review
- Article
- Myelin histology: a key tool in nervous system research.Neural regeneration research · 2024Review
- Dermoscopic and Histopathologic Analysis of the Correlation between the Pigmentation of Basal Cell Carcinoma and Tumor Aggressiveness.Annals of dermatology · 2023Article
- Amber photobiomodulation versus tranexamic acid for the treatment of melasma: protocol for a double-blind, randomised controlled trial.BMJ open · 2023Article
- Article
- Melanin concentration maps by label-free super-resolution photo-thermal imaging on melanoma biopsies.Biomedical optics express · 2022Article
- Basic Quality Controls Used in Skin Tissue Engineering.Life (Basel, Switzerland) · 2021Review
- Article
- Spatial mapping of the collagen distribution in human and mouse tissues by force volume atomic force microscopy.Scientific reports · 2020Article
- Label-Free Nanoimaging of Neuromelanin in the Brain by Soft X-ray Spectromicroscopy.Angewandte Chemie (International ed. in English) · 2020Article
- Slide-free clinical imaging of melanin with absolute quantities using label-free third-harmonic-generation enhancement-ratio microscopy.Biomedical optics express · 2020Article
- Evaluation of Fibrin-Agarose Tissue-Like Hydrogels Biocompatibility for Tissue Engineering Applications.Frontiers in bioengineering and biotechnology · 2020Article
- Local Growth Hormone Therapy for Pressure Ulcer Healing on a Human Skin Mouse Model.International journal of molecular sciences · 2019Article
- Characterization of the human ridged and non-ridged skin: a comprehensive histological, histochemical and immunohistochemical analysis.Histochemistry and cell biology · 2019Article
- Wharton's jelly-derived mesenchymal cells as a new source for the generation of microtissues for tissue engineering applications.Histochemistry and cell biology · 2018Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For the histopathologic diagnosis of melanocytic lesions, it could be necessary to identify the melanin pigment because its visualization is unspecific with hematoxylin-eosin (HE). The Fontana-Masson (FM) technique is used in histopathology in this type of lesion, which allows the identification of the pigment, but it loses all the morphologic parameters. The authors describe a modification to the FM method, for the evaluation of the morphology, the argentaffin reaction of the melanin, and collagens fibers of the extracellular matrix simultaneously, for which they have developed the Fontana-Masson picrosirius (FMPS) method. Biopsies of different melanocytic lesions were used for the performance of the HE, FM, and FMPS methods. The pixel intensity of the reaction for melanin, collagen, and epithelium was determined with ImageJ software. The FMPS method allows the evaluation of morphological characteristics, identifying the melanin pigment and collagen fibers with high intensity simultaneously. This method does not differ significantly from FM in the identification of melanin, maintaining its sensitivity and specificity. In addition, it does not differ in the demonstration of the morphology with HE. However, FMPS is significantly superior in the identification of collagen fibers. The FMPS method combines morphological and histochemical parameters that could be useful in the study of pigmented lesions of melanocytic origin.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.