ArticleJournal of bacteriology2011
Absence of PmrAB-mediated phosphoethanolamine modifications of Citrobacter rodentium lipopolysaccharide affects outer membrane integrity.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Phosphoethanolamine-modified lipid A enables antimicrobial resistance and atypical signaling properties in the marine pathogenMicrobiology spectrum · 2026Article
- The multifaceted roles of phosphoethanolamine-modified lipopolysaccharides: from stress response and virulence to cationic antimicrobial resistance.Microbiology and molecular biology reviews : MMBR · 2024Review
- Conserved patterns of sequence diversification provide insight into the evolution of two-component systems inMicrobial genomics · 2024Article
- Membrane properties modulation by SanA: implications for xenobiotic resistance inFrontiers in microbiology · 2023Article
- Functional Mechanism of Antimicrobial Peptide Bomidin and Its Safety for Macrobrachium rosenbergii.Probiotics and antimicrobial proteins · 2022Article
- The Characterization of Two-Component System PmrA/PmrB inFrontiers in microbiology · 2020Article
- PmrC (EptA) and CptA Negatively Affect Outer Membrane Vesicle Production in Citrobacter rodentium.Journal of bacteriology · 2019Article
- Identification of EnvC and Its Cognate Amidases as Novel Determinants of Intrinsic Resistance to Cationic Antimicrobial Peptides.Antimicrobial agents and chemotherapy · 2016Article
- Regulation of bacterial virulence by Csr (Rsm) systems.Microbiology and molecular biology reviews : MMBR · 2015Review
- LPS modification promotes maintenance of Yersinia pestis in fleas.Microbiology (Reading, England) · 2015Article
- Top-Down Strategies for the Structural Elucidation of Intact Gram-negative Bacterial Endotoxins.Chemical science · 2014Article
- Microbial modulation of host immunity with the small molecule phosphorylcholine.Infection and immunity · 2013Review
- The biology of the PmrA/PmrB two-component system: the major regulator of lipopolysaccharide modifications.Annual review of microbiology · 2013Review
- Reciprocal control between a bacterium's regulatory system and the modification status of its lipopolysaccharide.Molecular cell · 2012Article
- Phosphorylcholine allows for evasion of bactericidal antibody by Haemophilus influenzae.PLoS pathogens · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The PmrAB two-component system of enterobacteria regulates a number of genes whose protein products modify lipopolysaccharide (LPS). The LPS is modified during transport to the bacterial outer membrane (OM). A subset of PmrAB-mediated LPS modifications consists of the addition of phosphoethanolamine (pEtN) to lipid A by PmrC and to the core by CptA. In Salmonella enterica, pEtN modifications have been associated with resistance to polymyxin B and to excess iron. To investigate putative functions of pEtN modifications in Citrobacter rodentium, ΔpmrAB, ΔpmrC, ΔcptA, and ΔpmrC ΔcptA deletion mutants were constructed. Compared to the wild type, most mutant strains were found to be more susceptible to antibiotics that must diffuse across the LPS layer of the OM. All mutant strains also showed increased influx rates of ethidium dye across their OM, suggesting that PmrAB-regulated pEtN modifications affect OM permeability. This was confirmed by increased partitioning of the fluorescent dye 1-N-phenylnaphthylamine (NPN) into the OM phospholipid layer of the mutant strains. In addition, substantial release of periplasmic β-lactamase was observed for the ΔpmrAB and ΔpmrC ΔcptA strains, indicating a loss of OM integrity. This study attributes a new role for PmrAB-mediated pEtN LPS modifications in the maintenance of C. rodentium OM integrity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.