ArticleJournal of bacteriology2011
Activity of the Rhodopseudomonas palustris p-coumaroyl-homoserine lactone-responsive transcription factor RpaR.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- Synthetic microbe-to-plant communication channels.Nature communications · 2024Article
- AmBio · 2023Article
- Quorum Sensing Is Required for the Colony Establishment of a Plant Phyllosphere Bacterium Rhodopseudomonas palustris Strain GJ-22.Applied and environmental microbiology · 2023Article
- Recent Advances in the Mechanisms and Regulation of QS in Dairy Spoilage byFoods (Basel, Switzerland) · 2021Review
- Improved bacterial RNA-seq by Cas9-based depletion of ribosomal RNA reads.RNA (New York, N.Y.) · 2020Article
- Towards the Response Threshold forApplied and environmental microbiology · 2018Article
- An aryl-homoserine lactone quorum-sensing signal produced by a dimorphic prosthecate bacterium.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Tools for engineering coordinated system behaviour in synthetic microbial consortia.Nature communications · 2018Article
- Non-ribosomal Peptide Synthases from Pseudomonas aeruginosa Play a Role in Cyclodipeptide Biosynthesis, Quorum-Sensing Regulation, and Root Development in a Plant Host.Microbial ecology · 2017Article
- Article
- Quorum Sensing Communication Modules for Microbial Consortia.ACS synthetic biology · 2016Article
- Article
- Potential Emergence of Multi-quorum Sensing Inhibitor Resistant (MQSIR) Bacteria.Indian journal of microbiology · 2016Review
- Evolution of KaiC-Dependent Timekeepers: A Proto-circadian Timing Mechanism Confers Adaptive Fitness in the Purple Bacterium Rhodopseudomonas palustris.PLoS genetics · 2016Article
- Production of phenylacetyl-homoserine lactone analogs by artificial biosynthetic pathway in Escherichia coli.Microbial cell factories · 2015Article
- BadR and BadM Proteins Transcriptionally Regulate Two Operons Needed for Anaerobic Benzoate Degradation by Rhodopseudomonas palustris.Applied and environmental microbiology · 2015Article
- A solo luxI-type gene directs acylhomoserine lactone synthesis and contributes to motility control in the marine sponge symbiont Ruegeria sp. KLH11.Microbiology (Reading, England) · 2015Article
- Non-growing Rhodopseudomonas palustris increases the hydrogen gas yield from acetate by shifting from the glyoxylate shunt to the tricarboxylic acid cycle.The Journal of biological chemistry · 2014Article
- Non-native N-aroyl L-homoserine lactones are potent modulators of the quorum sensing receptor RpaR in Rhodopseudomonas palustris.Chembiochem : a European journal of chemical biology · 2014Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Rhodopseudomonas palustris transcriptional regulator RpaR responds to the RpaI-synthesized quorum-sensing signal p-coumaroyl-homoserine lactone (pC-HSL). Other characterized RpaR homologs respond to fatty acyl-HSLs. We show here that RpaR functions as a transcriptional activator, which binds directly to the rpaI promoter. We developed an RNAseq method that does not require a ribosome depletion step to define a set of transcripts regulated by pC-HSL and RpaR. The transcripts include several noncoding RNAs. A footprint analysis showed that purified His-tagged RpaR (His(6)-RpaR) binds to an inverted repeat element centered 48.5 bp upstream of the rpaI transcript start site, which we mapped by S1 nuclease protection and primer extension analyses. Although pC-HSL-RpaR bound to rpaI promoter DNA, it did not bind to the promoter regions of a number of RpaR-regulated genes not in the rpaI operon. This indicates that RpaR control of these other genes is indirect. Because the RNAseq analysis allowed us to track transcript strand specificity, we discovered that there is pC-HSL-RpaR-activated antisense transcription of rpaR. These data raise the possibility that this antisense RNA or other RpaR-activated noncoding RNAs mediate the indirect activation of genes in the RpaR-controlled regulon.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.