ArticleJournal of bacteriology2011
Amino acid residues in the GerAB protein important in the function and assembly of the alanine spore germination receptor of Bacillus subtilis 168.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 47 citations in OpenAlex.
- GerAB residues predicted to interact with water based on MD simulations mediate germinosome stability inFrontiers in microbiology · 2026Article
- Recent progress in proteins regulating the germination ofJournal of bacteriology · 2025Review
- GerAB residues predicted to interfere with water passage based on steered molecular dynamics are key to germinosome functionality.Frontiers in microbiology · 2025Article
- L-Alanine promotes anti-infectious properties of Bacillus subtilis S-2 spores via the germination receptor gerAA.Probiotics and antimicrobial proteins · 2024Article
- SpoVAF and FigP assemble into oligomeric ion channels that enhance spore germination.Genes & development · 2024Article
- A Family of Spore Lipoproteins Stabilizes the Germination Apparatus by Altering Inner Spore Membrane Fluidity inJournal of bacteriology · 2023Article
- Dormant spores sense amino acids through the B subunits of their germination receptors.Nature communications · 2021Article
- Predicting the Structure and Dynamics of Membrane Protein GerAB fromInternational journal of molecular sciences · 2021Article
- Importance of Individual Germination Receptor Subunits in the Cooperative Function between GerA and Ynd.Journal of bacteriology · 2019Article
- Structural and functional analyses of the N-terminal domain of the A subunit of aProceedings of the National Academy of Sciences of the United States of America · 2019Article
- The orphan germinant receptor protein GerXAO (but not GerX3b) is essential for L-alanine induced germination in Clostridium botulinum Group II.Scientific reports · 2018Article
- Phenotypic memory in Bacillus subtilis links dormancy entry and exit by a spore quantity-quality tradeoff.Nature communications · 2018Article
- The Bacillus subtilis germinant receptor GerA triggers premature germination in response to morphological defects during sporulation.Molecular microbiology · 2017Article
- Spore Heat Activation Requirements and Germination Responses Correlate with Sequences of Germinant Receptors and with the Presence of a SpecificApplied and environmental microbiology · 2017Article
- Analysis of Germination Capacity and Germinant Receptor (Sub)clusters of Genome-Sequenced Bacillus cereus Environmental Isolates and Model Strains.Applied and environmental microbiology · 2017Article
- Diversity of the Germination Apparatus inFrontiers in microbiology · 2016Article
- Bacillus thermoamylovorans Spores with Very-High-Level Heat Resistance Germinate Poorly in Rich Medium despite the Presence of ger Clusters but Efficiently upon Exposure to Calcium-Dipicolinic Acid.Applied and environmental microbiology · 2015Article
- Identification of a Novel Lipoprotein Regulator of Clostridium difficile Spore Germination.PLoS pathogens · 2015Article
- Expansion of the APC superfamily of secondary carriers.Proteins · 2014Article
- L-alanine-induced germination in Bacillus licheniformis -the impact of native gerA sequences.BMC microbiology · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The paradigm gerA operon is required for endospore germination in response to c-alanine as the sole germinant, and the three protein products, GerAA, GerAB, and GerAC are predicted to form a receptor complex in the spore inner membrane. GerAB shows homology to the amino acid-polyamine-organocation (APC) family of single-component transporters and is predicted to be an integral membrane protein with 10 membrane-spanning helices. Site-directed mutations were introduced into the gerAB gene at its natural location on the chromosome. Alterations to some charged or potential helix-breaking residues within membrane spans affected receptor function dramatically. In some cases, this is likely to reflect the complete loss of the GerA receptor complex, as judged by the absence of the germinant receptor protein GerAC, which suggests that the altered GerAB protein itself may be unstable or that the altered structure destabilizes the complex. Mutants that have a null phenotype for Instituto de Biotecnología de León, INBIOTEC, Parque Científico de León, Av. Real, 1, 24006 León, Spain-alanine germination but retain GerAC protein at near-normal levels are more likely to define amino acid residues of functional, rather than structural, importance. Single-amino-acid substitutions in each of the GerAB and GerAA proteins can prevent incorporation of GerAC protein into the spore; this provides strong evidence that the proteins within a specific receptor interact and that these interactions are required for receptor assembly. The lipoprotein nature of the GerAC receptor subunit is also important; an amino acid change in the prelipoprotein signal sequence in the gerAC1 mutant results in the absence of GerAC protein from the spore.
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