ArticleThe Journal of biological chemistry2011
G-actin participates in RNA polymerase II-dependent transcription elongation by recruiting positive transcription elongation factor b (P-TEFb).
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 64 citations in OpenAlex.
- Feeling the force from within - new tools and insights into nuclear mechanotransduction.Journal of cell science · 2025Review
- Plasma membrane and nuclear phosphatidylinositol 4,5-bisphosphate signalling in cancer.Lipids in health and disease · 2025Review
- Bioinformatics Analysis of Actin Interactome: Characterization of the Nuclear and Cytoplasmic Actin-Binding Proteins.The protein journal · 2024Article
- Dynamic coalescence of yeast Heat Shock Protein genes bypasses the requirement for actin.Genetics · 2023Article
- Prostaglandins limit nuclear actin to control nucleolar function during oogenesis.Frontiers in cell and developmental biology · 2023Article
- Mechanical regulation of chromatin and transcription.Nature reviews. Genetics · 2022Review
- Actin dynamics in protein homeostasis.Bioscience reports · 2022Review
- Nuclear Actin Dynamics in Gene Expression, DNA Repair, and Cancer.Results and problems in cell differentiation · 2022Article
- Mechanical Regulation of Transcription: Recent Advances.Trends in cell biology · 2021Review
- Article
- New Insights into Cellular Functions of Nuclear Actin.Biology · 2021Review
- Emerging Properties and Functions of Actin and Actin Filaments Inside the Nucleus.Cold Spring Harbor perspectives in biology · 2021Review
- Glucose and TNF enhance expression ofGene: X · 2020Article
- Nucleoskeletal regulation of transcription: Actin on MRTF.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- RASSF1A is required for the maintenance of nuclear actin levels.The EMBO journal · 2019Article
- Lamin A/C and Emerin depletion impacts chromatin organization and dynamics in the interphase nucleus.BMC molecular and cell biology · 2019Article
- Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing.Journal of cell science · 2019Article
- Nuclear Actin: From Discovery to Function.Anatomical record (Hoboken, N.J. : 2007) · 2018Review
- Article
- Nuclear actin: ancient clue to evolution in eukaryotes?Histochemistry and cell biology · 2018Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling.
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