ArticleNucleic acids research2011
Segrosome assembly at the pliable parH centromere.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- A telomere-to-telomere reference genome assembly of the Hypomesus nipponensis.Scientific data · 2026Article
- Insights into the molecular mechanism of ParABS system in chromosome partition by HpParA and HpParB.Nucleic acids research · 2024Article
- Telomere-to-telomere genome assembly of the goose Anser cygnoides.Scientific data · 2024Article
- Genome sequencing and comparative analysis of Wolbachia strain wAlbA reveals Wolbachia-associated plasmids are common.PLoS genetics · 2022Article
- Genome Segregation by the Venus Flytrap Mechanism: Probing the Interaction Between the ParF ATPase and the ParG Centromere Binding Protein.Frontiers in molecular biosciences · 2020Article
- Plasmid Localization and Partition inEcoSal Plus · 2019Review
- A three-dimensional ParF meshwork assembles through the nucleoid to mediate plasmid segregation.Nucleic acids research · 2017Article
- Segrosome Complex Formation during DNA Trafficking in Bacterial Cell Division.Frontiers in molecular biosciences · 2016Review
- Breaking and restoring the hydrophobic core of a centromere-binding protein.The Journal of biological chemistry · 2015Article
- pA506, a conjugative plasmid of the plant epiphyte Pseudomonas fluorescens A506.Applied and environmental microbiology · 2013Article
- Uncoupling of nucleotide hydrolysis and polymerization in the ParA protein superfamily disrupts DNA segregation dynamics.The Journal of biological chemistry · 2012Article
- Combinatorial targeting of ribbon-helix-helix artificial transcription factors to chimeric recognition sites.Nucleic acids research · 2012Article
- Structural mechanism of ATP-induced polymerization of the partition factor ParF: implications for DNA segregation.The Journal of biological chemistry · 2012Article
- Centromere binding and evolution of chromosomal partition systems in the Burkholderiales.Journal of bacteriology · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The segrosome of multiresistance plasmid TP228 comprises ParF, which is a member of the ParA ATPase superfamily, and the ParG ribbon-helix-helix factor that assemble jointly on the parH centromere. Here we demonstrate that the distinctive parH site (∼100-bp) consists of an array of degenerate tetramer boxes interspersed by AT-rich spacers. Although numerous consecutive AT-steps are suggestive of inherent curvature, parH lacks an intrinsic bend. Sequential deletion of parH tetramers progressively reduced centromere function. Nevertheless, the variant subsites could be rearranged in different geometries that accommodated centromere activity effectively revealing that the site is highly elastic in vivo. ParG cooperatively coated parH: proper centromere binding necessitated the protein's N-terminal flexible tails which modulate the centromere binding affinity of ParG. Interaction of the ParG ribbon-helix-helix domain with major groove bases in the tetramer boxes likely provides direct readout of the centromere. In contrast, the AT-rich spacers may be implicated in indirect readout that mediates cooperativity between ParG dimers assembled on adjacent boxes. ParF alone does not bind parH but instead loads into the segrosome interactively with ParG, thereby subtly altering centromere conformation. Assembly of ParF into the complex requires the N-terminal flexible tails in ParG that are contacted by ParF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.