ArticleJournal of experimental botany2011
In vivo protein tyrosine nitration in Arabidopsis thaliana.
Article in Journal of experimental botany, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
70 citing papers in PubMed, 199 citations in OpenAlex.
- Article
- Integration of NO and HPlant communications · 2026Review
- Peroxynitrite-mediated tyrosine nitration modulates β-1,3-glucanase activity and potato defense againstFrontiers in plant science · 2026Article
- Dynamic interplay of reactive oxygen and nitrogen species (ROS and RNS) in plant resilience: unveiling the signaling pathways and metabolic responses to biotic and abiotic stresses.Plant cell reports · 2024Review
- Article
- Reactive oxygen species- and nitric oxide-dependent regulation of ion and metal homeostasis in plants.Journal of experimental botany · 2023Article
- Nitric Oxide Acts as a Key Signaling Molecule in Plant Development under Stressful Conditions.International journal of molecular sciences · 2023Review
- The role of nitric oxide and hydrogen sulfide in regulation of redox homeostasis at extreme temperatures in plants.Frontiers in plant science · 2023Review
- Redox status of the plant cell determines epigenetic modifications under abiotic stress conditions and during developmental processes.Journal of advanced research · 2022Review
- Peroxisome-Mediated Reactive Oxygen Species Signals Modulate Programmed Cell Death in Plants.International journal of molecular sciences · 2022Review
- Nitric Oxide (NO) Differentially Modulates the Ascorbate Peroxidase (APX) Isozymes of Sweet Pepper (Antioxidants (Basel, Switzerland) · 2022Article
- Insights into the expression of DNA (de)methylation genes responsive to nitric oxide signaling in potato resistance to late blight disease.Frontiers in plant science · 2022Article
- Plant homocysteine, a methionine precursor and plant's hallmark of metabolic disorders.Frontiers in plant science · 2022Review
- Protein Tyrosine Nitration in Plant Nitric Oxide Signaling.Frontiers in plant science · 2022Review
- Peroxisomes as redox-signaling nodes in intracellular communication and stress responses.Plant physiology · 2021Review
- Nitric oxide function during oxygen deprivation in physiological and stress processes.Journal of experimental botany · 2021Review
- Interactions between metabolism and chromatin in plant models.Molecular metabolism · 2020Review
- Matrix Redox Physiology Governs the Regulation of Plant Mitochondrial Metabolism through Posttranslational Protein Modifications.The Plant cell · 2020Review
- Redox Components: Key Regulators of Epigenetic Modifications in Plants.International journal of molecular sciences · 2020Review
- Multilevel Regulation of Peroxisomal Proteome by Post-Translational Modifications.International journal of molecular sciences · 2019Review
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitration of tyrosine (Y) residues of proteins is a low abundant post-translational modification that modulates protein function or fate in animal systems. However, very little is known about the in vivo prevalence of this modification and its corresponding targets in plants. Immunoprecipitation, based on an anti-3-nitroY antibody, was performed to pull-down potential in vivo targets of Y nitration in the Arabidopsis thaliana proteome. Further shotgun liquid chromatography-mass spectrometry (LC-MS/MS) proteomic analysis of the immunoprecipitated proteins allowed the identification of 127 proteins. Around 35% of them corresponded to homologues of proteins that have been previously reported to be Y nitrated in other non-plant organisms. Some of the putative in vivo Y-nitrated proteins were further confirmed by western blot with specific antibodies. Furthermore, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) analysis of protein spots, separated by two-dimensional electrophoresis from immunoprecipitated proteins, led to the identification of seven nitrated peptides corresponding to six different proteins. However, in vivo nitration sites among putative targets could not be identified by MS/MS. Nevertheless, an MS/MS spectrum with 3-aminoY318 instead of the expected 3-nitroY was found for cytosolic glyceraldehyde-3-phosphate dehydrogenase. Reduction of nitroY to aminoY during MS-based proteomic analysis together with the in vivo low abundance of these modifications made the identification of nitration sites difficult. In turn, in vitro nitration of methionine synthase, which was also found in the shotgun proteomic screening, allowed unequivocal identification of a nitration site at Y287.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.