ArticleApplied and environmental microbiology2011
LacI(Ts)-regulated expression as an in situ intracellular biomolecular thermometer.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Intracellular thermometry with fluorescent sensors for thermal biology.Pflugers Archiv : European journal of physiology · 2018Review
- Beyond the bulk: disclosing the life of single microbial cells.FEMS microbiology reviews · 2017Review
- Design and development of genetically encoded fluorescent sensors to monitor intracellular chemical and physical parameters.Biophysical reviews · 2016Review
- A critique of methods for temperature imaging in single cells.Nature methods · 2014Article
- Bacterial RNA thermometers: molecular zippers and switches.Nature reviews. Microbiology · 2012Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
In response to needs for in situ thermometry, a temperature-sensitive vector was adapted to report changes in the intracellular heat content of Escherichia coli in near-real time. This model system utilized vectors expressing increasing quantities of β-galactosidase in response to stepwise temperature increases through a biologically relevant range (22 to 45°C). As judged by calibrated fluorometric and colorimetric reporters, both whole E. coli cells and lysates expressed significant repeatable changes in β-galactosidase activity that were sensitive to temperature changes of less than 1°C (35 to 45°C). This model system suggests that changes in cellular heat content can be detected independently of the medium in which cells are maintained, a feature of particular importance where the medium is heterogeneous or nonaqueous, or otherwise has a low heat transfer capacity. We report here that the intracellular temperature can be reliably obtained in near-real time using reliable fluorescent reporting systems from cellular scales, with a 20°C range of detection and at least 0.7°C sensitivity between 35 and 45°C.
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Registered trials
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