ArticleApplied and environmental microbiology2011
Fungal gene expression on demand: an inducible, tunable, and metabolism-independent expression system for Aspergillus niger.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers, 1 of them a synthesis that pooled it.
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Who cites it
94 citing papers in PubMed, 1 synthesis or guideline pooled it, 253 citations in OpenAlex.
- Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.Nucleic acids research · 2019Pooled it
- Genome characterization of a novel chrysovirus infecting Aspergillus Niger.Archives of virology · 2026Article
- Fungal Innovations-Advancing Sustainable Materials, Genetics, and Applications for Industry.Journal of fungi (Basel, Switzerland) · 2025Review
- The multipurpose cell factory Aspergillus niger can be engineered to produce hydroxylated collagen.Biotechnology for biofuels and bioproducts · 2025Article
- Global survey of secondary metabolism inPNAS nexus · 2025Article
- Recent Advances in Heterologous Protein Expression and Natural Product Synthesis byJournal of fungi (Basel, Switzerland) · 2025Review
- The Tetracycline-Inducible/CRISPR-Cas9 System is an Efficient Tool for Studying Gene Function in Phytophthora sojae.Molecular plant pathology · 2025Article
- Cell walls of filamentous fungi - challenges and opportunities for biotechnology.Applied microbiology and biotechnology · 2025Review
- Overexpression of the RNA-binding protein NrdA affects global gene expression and secondary metabolism inmSphere · 2025Article
- The palmitoyl-CoA ligase Fum16 is part of amBio · 2025Article
- Promoter engineering with programmable upstream activating sequences in Aspergillus Niger cell factory.Microbial cell factories · 2025Article
- Harnessing the Tet-Off system for effective gene silencing inFrontiers in plant science · 2025Article
- Article
- Tuning fungal promoters for the expression of eukaryotic proteins.World journal of microbiology & biotechnology · 2024Review
- A novel luciferase-based reporter tool to monitor the dynamics of carbon catabolite repression in filamentous fungi.Microbial biotechnology · 2024Article
- The Tet-on system for controllable gene expression in the rock-inhabiting black fungus Knufia petricola.Extremophiles : life under extreme conditions · 2024Article
- NHEJ and HDR can occur simultaneously during gene integration into the genome of Aspergillus niger.Fungal biology and biotechnology · 2024Article
- Enhanced surface colonisation and competition during bacterial adaptation to a fungus.Nature communications · 2024Article
- The antifungal peptide AnAFP fromFrontiers in microbiology · 2024Article
- Establishing a one-step marker-free CRISPR/Cas9 system for industrial Aspergillus niger using counter-selectable marker Ang-ace2.Biotechnology letters · 2023Article
34 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Filamentous fungi are the cause of serious human and plant diseases but are also exploited in biotechnology as production platforms. Comparative genomics has documented their genetic diversity, and functional genomics and systems biology approaches are under way to understand the functions and interaction of fungal genes and proteins. In these approaches, gene functions are usually inferred from deletion or overexpression mutants. However, studies at these extreme points give only limited information. Moreover, many overexpression studies use metabolism-dependent promoters, often causing pleiotropic effects and thus limitations in their significance. We therefore established and systematically evaluated a tunable expression system for Aspergillus niger that is independent of carbon and nitrogen metabolism and silent under noninduced conditions. The system consists of two expression modules jointly targeted to a defined genomic locus. One module ensures constitutive expression of the tetracycline-dependent transactivator rtTA2(S)-M2, and one module harbors the rtTA2(S)-M2-dependent promoter that controls expression of the gene of interest (the Tet-on system). We show here that the system is tight, responds within minutes after inducer addition, and allows fine-tuning based on the inducer concentration or gene copy number up to expression levels higher than the expression levels of the gpdA promoter. We also validate the Tet-on system for the generation of conditional overexpression mutants and demonstrate its power when combined with a gene deletion approach. Finally, we show that the system is especially suitable when the functions of essential genes must be examined.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.