ArticleDevelopmental biology2011
Ascl3 knockout and cell ablation models reveal complexity of salivary gland maintenance and regeneration.
Article in Developmental biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 59 citations in OpenAlex.
- Development-inspired approach for the derivation of functional and transplantable salivary gland organoids from human pluripotent stem cells.Nature communications · 2026Article
- Potassium Channels of the Airway Epithelium.Acta physiologica (Oxford, England) · 2026Review
- Ascl3The Journal of physiology · 2026Article
- Article
- A mathematical model of ENaC and Slc26a6 regulation by CFTR in salivary gland ducts.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- Specification of distinct cell types in a sensory-adhesive organ important for metamorphosis in tunicate larvae.PLoS biology · 2024Article
- CFTR expression in human salivary gland acinar cells.American journal of physiology. Cell physiology · 2024Article
- Tlx1 regulates acinar and duct development in mouse salivary glands.Journal of anatomy · 2024Article
- Duct ligation/de-ligation model: exploring mechanisms for salivary gland injury and regeneration.Frontiers in cell and developmental biology · 2024Review
- Autologous mesenchymal stem cells offer a new paradigm for salivary gland regeneration.International journal of oral science · 2023Review
- Genetic Study of Elf5 and Ehf in the Mouse Salivary Gland.Journal of dental research · 2023Article
- Salivary gland function, development, and regeneration.Physiological reviews · 2022Review
- A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands.Cell reports · 2022Article
- Sci-Seq of Human Fetal Salivary Tissue Introduces Human Transcriptional Paradigms and a Novel Cell Population.Frontiers in dental medicine · 2022Article
- Cell signaling regulation in salivary gland development.Cellular and molecular life sciences : CMLS · 2021Review
- Regenerative Medicine of Epithelia: Lessons From the Past and Future Goals.Frontiers in bioengineering and biotechnology · 2021Review
- Stress or injury induces cellular plasticity in salivary gland acinar cells.Cell and tissue research · 2020Article
- Generation of functional salivary gland tissue from human submandibular gland stem/progenitor cells.Stem cell research & therapy · 2020Article
- Slc4a11 disruption causes duct cell loss and impairs NaCl reabsorption in female mouse submandibular glands.Physiological reports · 2019Article
- Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Expression of the transcription factor, Ascl3, marks a population of adult progenitor cells, which can give rise to both acinar and duct cell types in the murine salivary glands. Using a previously reported Ascl3(EGFP-Cre/+) knock-in strain, we demonstrate that Ascl3-expressing cells represent a molecularly distinct, and proliferating population of progenitor cells located in salivary gland ducts. To investigate both the role of the Ascl3 transcription factor, and the role of the cells in which it is expressed, we generated knockout and cell-specific ablation models. Ascl3 knockout mice develop smaller salivary glands than wild type littermates, but secrete saliva normally. They display a lower level of cell proliferation, consistent with their smaller size. In the absence of Ascl3, the cells maintain their progenitor function and continue to generate both acinar and duct cells. To directly test the role of the progenitor cells, themselves, in salivary gland development and regeneration, we used Cre-activated expression of diphtheria toxin (DTA) in the Ascl3-expressing (Ascl3+) cell population, resulting in specific cell ablation of Ascl3+ cells. In the absence of the Ascl3+ progenitor cells, the mice developed morphologically normal, albeit smaller, salivary glands able to secrete saliva. Furthermore, in a ductal ligation model of salivary gland injury, the glands of these mice were able to regenerate acinar cells. Our results indicate that Ascl3+ cells are active proliferating progenitors, but they are not the only precursors for salivary gland development or regeneration. We conclude that maintenance of tissue homeostasis in the salivary gland must involve more than one progenitor cell population.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.