ArticleExperimental neurology2011
The generation of olfactory epithelial neurospheres in vitro predicts engraftment capacity following transplantation in vivo.
Article in Experimental neurology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Systematic review of literature regarding the isolation of mesenchymal adult stem cells from the olfactory epithelium.Frontiers in cellular neuroscience · 2026Pooled it
- Strategies to Develop Regenerative Medicine Approaches for Olfactory Disorders.Clinical and experimental otorhinolaryngology · 2025Review
- Organoid models for chemosensing cell studies.American journal of physiology. Cell physiology · 2025Review
- Quiescent horizontal basal stem cells act as a niche for olfactory neurogenesis in a mouse 3D organoid model.Cell reports methods · 2025Article
- Granulocyte Colony Stimulating Factor (G-CSF) and Olfactory Function-A Clinical Pilot Study.Laryngoscope investigative otolaryngology · 2025Article
- Role and Mechanism of Olfactory Stem Cells in the Treatment of Olfactory Disorders.Stem cells international · 2025Review
- Axonal Regrowth of Olfactory Sensory Neurons In Vitro.International journal of molecular sciences · 2023Article
- Isolation and Differentiation of Neurons and Glial Cells from Olfactory Epithelium in Living Subjects.Molecular neurobiology · 2023Article
- The Fate of Transplanted Olfactory Progenitors Is Conditioned by the Cell Phenotypes of the Receiver Brain Tissue in Cocultures.International journal of molecular sciences · 2020Article
- Human olfactory mesenchymal stromal cells co-expressing horizontal basal and ensheathing cell proteins in culture.Biomedica : revista del Instituto Nacional de Salud · 2020Article
- Activating a Reserve Neural Stem Cell Population In Vitro Enables Engraftment and Multipotency after Transplantation.Stem cell reports · 2019Article
- Spheroid Culture of Mammalian Olfactory Receptor Neurons: Potential Applications for a Bioelectronic Nose.Experimental neurobiology · 2018Article
- Topical Dexamethasone Administration Impairs Protein Synthesis and Neuronal Regeneration in the Olfactory Epithelium.Frontiers in molecular neuroscience · 2018Article
- Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.Cell stem cell · 2017Article
- Isolation, culture optimization and functional characterization of stem cell neurospheres from mouse neonatal olfactory bulb and epithelium.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2017Article
- Stem and progenitor cells of the mammalian olfactory epithelium: Taking poietic license.The Journal of comparative neurology · 2017Review
- Contribution of Polycomb group proteins to olfactory basal stem cell self-renewal in a novel c-KIT+ culture model and in vivo.Development (Cambridge, England) · 2016Article
- Translational potential of olfactory mucosa for the study of neuropsychiatric illness.Translational psychiatry · 2015Review
- Adult c-Kit(+) progenitor cells are necessary for maintenance and regeneration of olfactory neurons.The Journal of comparative neurology · 2015Article
- Wnt-responsive Lgr5⁺ globose basal cells function as multipotent olfactory epithelium progenitor cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The stem and progenitor cells of the olfactory epithelium maintain the tissue throughout life and effectuate epithelial reconstitution after injury. We have utilized free-floating olfactory neurosphere cultures to study factors influencing proliferation, differentiation, and transplantation potency of sphere-grown cells as a first step toward using them for therapeutic purposes. Olfactory neurospheres form best and expand most when grown from neonatal epithelium, although methyl bromide-injured or normal adult material is weakly spherogenic. The spheres contain the full range of epithelial cell types as marked by cytokeratins, neuron-specific antigens, E-cadherin, Sox2, and Sox9. Globose basal cells are also prominent constituents. Medium conditioned by growth of phorbol ester-stimulated, immortalized lamina propria-derived cells (LP(Imm)) significantly increases the percentage of Neurog1eGFP(+) progenitors and immature neurons in spheres. Sphere-forming capacity resides within selected populations; FACS-purified, Neurog1eGFP(+) cells were poorly spherogenic, while preparations from ΔSox2eGFP transgenic mice that are enriched for Sox2(+) basal cells formed spheres very efficiently. Finally, we compared the potency following transplantation of cells grown in spheres vs. cells derived from adherent cultures. The sphere-derived cells engrafted and produced colonies with multiple cell types that incorporated into and resembled host epithelium; cells from adherent cultures did not. Furthermore, cells from spheres grown in conditioned media from the phorbol ester-activated LP(Imm) line gave rise to significantly more neurons after transplantation as compared with control. The current findings demonstrate that sphere formation serves as a biomarker for engraftment capacity and multipotency of olfactory progenitors, which are requirements for their eventual translational use.
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