ArticleBMC genomics2011
Advancing Eucalyptus genomics: identification and sequencing of lignin biosynthesis genes from deep-coverage BAC libraries.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed.
- From genome to gene expression: the genomic landscape of a hybrid species of Eucalyptus urophylla × Eucalyptus grandis and its divergence from parental species hybrid.BMC plant biology · 2025Article
- Transcriptome analysis of the transition from primary to secondary growth of vertical stem in Eucalyptus grandis.BMC plant biology · 2024Article
- Enhancing prediction accuracy of foliar essential oil content, growth, and stem quality inFrontiers in plant science · 2024Article
- Identification of WUSCHEL-related homeobox gene and truncated small peptides in transformation efficiency improvement in Eucalyptus.BMC plant biology · 2023Article
- Genome survey and development of 13 SSR markers inJournal of genetics · 2022Article
- Comparison of eight complete chloroplast genomes of the endangered Aquilaria tree species (Thymelaeaceae) and their phylogenetic relationships.Scientific reports · 2020Article
- Landscape drivers of genomic diversity and divergence in woodland Eucalyptus.Molecular ecology · 2019Article
- Comprehensive Analysis ofPlants (Basel, Switzerland) · 2019Article
- The plastid and mitochondrial genomes of Eucalyptus grandis.BMC genomics · 2019Article
- Identifying genetic markers for a range of phylogenetic utility-From species to family level.PloS one · 2019Article
- The Koala (PeerJ · 2019Article
- Effective BAC clone anchoring with genotyping-by-sequencing and Diversity Arrays Technology in a large genome cereal rye.Scientific reports · 2018Article
- Chloroplast variation is incongruent with classification of the Australian bloodwood eucalypts (genus Corymbia, family Myrtaceae).PloS one · 2018Article
- Article
- EUCANEXT: an integrated database for the exploration of genomic and transcriptomic data from Eucalyptus species.Database : the journal of biological databases and curation · 2017Article
- Construction of co-expression network based on natural expression variation of xylogenesis-related transcripts in Eucalyptus tereticornis.Molecular biology reports · 2016Article
- Advancing Eucalyptus Genomics: Cytogenomics Reveals Conservation of Eucalyptus Genomes.Frontiers in plant science · 2016Article
- Plastid DNA insertions in plant nuclear genomes: the sites, abundance and ages, and a predicted promoter analysis.Functional & integrative genomics · 2015Review
- Structure, expression profile and phylogenetic inference of chalcone isomerase-like genes from the narrow-leafed lupin (Lupinus angustifolius L.) genome.Frontiers in plant science · 2015Article
- Development of genetic markers in Eucalyptus species by target enrichment and exome sequencing.PloS one · 2015Article
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15 authors.
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Abstract
backgroundEucalyptus species are among the most planted hardwoods in the world because of their rapid growth, adaptability and valuable wood properties. The development and integration of genomic resources into breeding practice will be increasingly important in the decades to come. Bacterial artificial chromosome (BAC) libraries are key genomic tools that enable positional cloning of important traits, synteny evaluation, and the development of genome framework physical maps for genetic linkage and genome sequencing.
resultsWe describe the construction and characterization of two deep-coverage BAC libraries EG_Ba and EG_Bb obtained from nuclear DNA fragments of E. grandis (clone BRASUZ1) digested with HindIII and BstYI, respectively. Genome coverages of 17 and 15 haploid genome equivalents were estimated for EG_Ba and EG_Bb, respectively. Both libraries contained large inserts, with average sizes ranging from 135 Kb (Eg_Bb) to 157 Kb (Eg_Ba), very low extra-nuclear genome contamination providing a probability of finding a single copy gene ≥ 99.99%. Libraries were screened for the presence of several genes of interest via hybridizations to high-density BAC filters followed by PCR validation. Five selected BAC clones were sequenced and assembled using the Roche GS FLX technology providing the whole sequence of the E. grandis chloroplast genome, and complete genomic sequences of important lignin biosynthesis genes.
conclusionsThe two E. grandis BAC libraries described in this study represent an important milestone for the advancement of Eucalyptus genomics and forest tree research. These BAC resources have a highly redundant genome coverage (> 15×), contain large average inserts and have a very low percentage of clones with organellar DNA or empty vectors. These publicly available BAC libraries are thus suitable for a broad range of applications in genetic and genomic research in Eucalyptus and possibly in related species of Myrtaceae, including genome sequencing, gene isolation, functional and comparative genomics. Because they have been constructed using the same tree (E. grandis BRASUZ1) whose full genome is being sequenced, they should prove instrumental for assembly and gap filling of the upcoming Eucalyptus reference genome sequence.
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