ArticleBiochemistry2011
Coupling efficiency of rhodopsin and transducin in bicelles.
Article in Biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Local membrane charge regulates βNature communications · 2019Article
- A Conserved Hydrophobic Core in Gαi1 Regulates G Protein Activation and Release from Activated Receptor.The Journal of biological chemistry · 2016Article
- Amphipols in G protein-coupled receptor pharmacology: what are they good for?The Journal of membrane biology · 2014Review
- Rhodopsin TM6 can interact with two separate and distinct sites on arrestin: evidence for structural plasticity and multiple docking modes in arrestin-rhodopsin binding.Biochemistry · 2014Article
- From atomic structures to neuronal functions of g protein-coupled receptors.Annual review of neuroscience · 2013Review
- Biophysical characterization of membrane proteins in nanodiscs.Methods (San Diego, Calif.) · 2013Review
- When detergent meets bilayer: birth and coming of age of lipid bicelles.Progress in nuclear magnetic resonance spectroscopy · 2013Review
- Involvement of distinct arrestin-1 elements in binding to different functional forms of rhodopsin.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Probing the transmembrane structure and topology of microsomal cytochrome-p450 by solid-state NMR on temperature-resistant bicelles.Scientific reports · 2013Article
- Structure-function studies with G protein-coupled receptors as a paradigm for improving drug discovery and development of therapeutics.Biotechnology journal · 2012Review
- The magic of bicelles lights up membrane protein structure.Chemical reviews · 2012Review
- Modulation of the interaction between neurotensin receptor NTS1 and Gq protein by lipid.Journal of molecular biology · 2012Article
- Allosteric mechanisms of G protein-Coupled Receptor signaling: a structural perspective.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Crystallizing membrane proteins using lipidic bicelles.Methods (San Diego, Calif.) · 2011Review
- Preparation of an activated rhodopsin/transducin complex using a constitutively active mutant of rhodopsin.Biochemistry · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
G protein coupled receptors (GPCRs) can be activated by various extracellular stimuli, including hormones, peptides, odorants, neurotransmitters, nucleotides, or light. After activation, receptors interact with heterotrimeric G proteins and catalyze GDP release from the Gα subunit, the rate limiting step in G protein activation, to form a high affinity nucleotide-free GPCR-G protein complex. In vivo, subsequent GTP binding reduces affinity of the Gα protein for the activated receptor. In this study, we investigated the biochemical and structural characteristics of the prototypical GPCR, rhodopsin, and its signaling partner, transducin (G(t)), in bicelles to better understand the effects of membrane composition on high affinity complex formation, stability, and receptor mediated nucleotide release. Our results demonstrate that the high-affinity complex (rhodopsin-G(t)(empty)) forms more readily and has dramatically increased stability when rhodopsin is integrated into bicelles of a defined composition. We increased the half-life of functional complex to 1 week in the presence of negatively charged phospholipids. These data suggest that a membrane-like structure is an important contributor to the formation and stability of functional receptor-G protein complexes and can extend the range of studies that investigate properties of these complexes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.