ArticleEuropean biophysics journal : EBJ2011
Water flux through human aquaporin 1: inhibition by intracellular furosemide and maximal response with high osmotic gradients.
Article in European biophysics journal : EBJ, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed.
- Aquaporin membrane channels in the hepatobiliary tract: a model of complexity and clinical implications in health and disease.Internal and emergency medicine · 2026Review
- Distinctive roles of aquaporins and novel therapeutic opportunities against cancer.RSC medicinal chemistry · 2025Review
- Article
- Investigating post-traumatic syringomyelia and local fluid osmoregulation via a rat model.Fluids and barriers of the CNS · 2024Article
- Methazolamide Reduces the AQP5 mRNA Expression and Immune Cell Migration-A New Potential Drug in Sepsis Therapy?International journal of molecular sciences · 2024Article
- Mechanosensitive aquaporins.Biophysical reviews · 2023Review
- Cellular Distribution Pattern of tjp1 (ZO-1) in Xenopus laevis Oocytes Heterologously Expressing Claudins.The Journal of membrane biology · 2023Article
- Aquaporins: New players in breast cancer progression and treatment response.Frontiers in oncology · 2022Review
- Inhibitors of Mammalian Aquaporin Water Channels.International journal of molecular sciences · 2019Review
- Review
- Plant and animal aquaporins crosstalk: what can be revealed from distinct perspectives.Biophysical reviews · 2017Review
- Loop Diuretics Diminish Hemolysis Induced by α-Hemolysin from Escherichia coli.The Journal of membrane biology · 2017Article
- Detecting Aquaporin Function and Regulation.Frontiers in chemistry · 2016Review
- The central role of aquaporins in the pathophysiology of ischemic stroke.Frontiers in cellular neuroscience · 2015Review
- Aquaporins: important but elusive drug targets.Nature reviews. Drug discovery · 2014Review
- Molecular dynamics of water in the neighborhood of aquaporins.European biophysics journal : EBJ · 2013Review
- Aquaporin water channels in the nervous system.Nature reviews. Neuroscience · 2013Review
- Human AQP1 is a constitutively open channel that closes by a membrane-tension-mediated mechanism.Biophysical journal · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This work studies water permeability properties of human aquaporin 1 (hAQP1) expressed in Xenopus laevis oocyte membranes, applying a technique where cellular content is replaced with a known medium, with the possibility of measuring intracellular pressure. Consequences on water transport-produced by well-known anisotonic gradients and by the intracellular effect of probable aquaporin inhibitors-were tested. In this way, the specific intracellular inhibition of hAQP1 by the diuretic drug furosemide was demonstrated. In addition, experiments imposing anisotonic mannitol gradients with a constant ionic strength showed that the relationship between water flux and the applied mannitol gradient deflects from a perfect osmometer response when the gradient is higher than 150 mosmol kg (W) (-1) . These results would indicate that the passage of water molecules through hAQP1 may have a maximum rate. As a whole, this work demonstrates the technical advantage of controlling both intracellular pressure and medium composition in order to study biophysical properties of hAQP1, and contributes information on water channel behavior under osmotic challenges and the discovery of new inhibitors.
Indexed as
Identifiers
21373963What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.