ArticleMolecular biology of the cell2011
The spindle assembly function of Caenorhabditis elegans katanin does not require microtubule-severing activity.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 45 citations in OpenAlex.
- Meteorin Is a Novel Interaction Partner of p60-Katanin During Mitosis in HCT-116 Colorectal Cancer Cells.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Microtubule length correlates with spindle length in C. elegans meiosis.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Microtubule-binding domains in Katanin p80 subunit are essential for severing activity in C. elegans.The Journal of cell biology · 2024Article
- Auxin-induced degradation of the aurora A kinase, AIR-1, inmicroPublication biology · 2024Article
- Cohesin is required for meiotic spindle assembly independent of its role in cohesion in C. elegans.PLoS genetics · 2022Article
- Article
- Evidence for anaphase pulling forces during C. elegans meiosis.The Journal of cell biology · 2020Article
- Spherical spindle shape promotes perpendicular cortical orientation by preventing isometric cortical pulling on both spindle poles duringDevelopment (Cambridge, England) · 2019Article
- Microtubule-severing enzymes: From cellular functions to molecular mechanism.The Journal of cell biology · 2018Review
- Structural basis of katanin p60:p80 complex formation.Scientific reports · 2017Article
- Katanin spiral and ring structures shed light on power stroke for microtubule severing.Nature structural & molecular biology · 2017Article
- Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex.Nature cell biology · 2017Article
- Assembly of Caenorhabditis elegans acentrosomal spindles occurs without evident microtubule-organizing centers and requires microtubule sorting by KLP-18/kinesin-12 and MESP-1.Molecular biology of the cell · 2016Article
- Regulation of the MEI-1/MEI-2 Microtubule-Severing Katanin Complex in Early Caenorhabditis elegans Development.G3 (Bethesda, Md.) · 2016Article
- A novel chromosome segregation mechanism during female meiosis.Molecular biology of the cell · 2016Article
- Proteomic Analysis of the Mammalian Katanin Family of Microtubule-severing Enzymes Defines Katanin p80 subunit B-like 1 (KATNBL1) as a Regulator of Mammalian Katanin Microtubule-severing.Molecular & cellular proteomics : MCP · 2016Article
- Article
- Oocyte Meiotic Spindle Assembly and Function.Current topics in developmental biology · 2016Review
- A novel family of katanin-like 2 protein isoforms (KATNAL2), interacting with nucleotide-binding proteins Nubp1 and Nubp2, are key regulators of different MT-based processes in mammalian cells.Cellular and molecular life sciences : CMLS · 2016Article
- Dynactin-dependent cortical dynein and spherical spindle shape correlate temporally with meiotic spindle rotation in Caenorhabditis elegans.Molecular biology of the cell · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Katanin is a heterodimeric microtubule-severing protein that is conserved among eukaryotes. Loss-of-function mutations in the Caenorhabditis elegans katanin catalytic subunit, MEI-1, cause specific defects in female meiotic spindles. To determine the relationship between katanin's microtubule-severing activity and its role in meiotic spindle formation, we analyzed the MEI-1(A338S) mutant. Unlike wild-type MEI-1, which mediated disassembly of microtubule arrays in Xenopus fibroblasts, MEI-1(A338S) had no effect on fibroblast microtubules, indicating a lack of microtubule-severing activity. In C. elegans, MEI-1(A338S) mediated assembly of extremely long bipolar meiotic spindles. In contrast, a nonsense mutation in MEI-1 caused assembly of meiotic spindles without any poles as assayed by localization of the spindle-pole protein, ASPM-1. These results indicated that katanin protein, but not katanin's microtubule-severing activity, is required for assembly of acentriolar meiotic spindle poles. To understand the nonsevering activities of katanin, we characterized the N-terminal domain of the katanin catalytic subunit. The N-terminal domain was necessary and sufficient for binding to the katanin regulatory subunit. The katanin regulatory subunit in turn caused a dramatic change in the microtubule-binding properties of the N-terminal domain of the catalytic subunit. This unique bipartite microtubule-binding structure may mediate the spindle-pole assembly activity of katanin during female meiosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.