ArticleMolecular endocrinology (Baltimore, Md.)2011
Gonadotropin-releasing hormone induces miR-132 and miR-212 to regulate cellular morphology and migration in immortalized LbetaT2 pituitary gonadotrope cells.
Article in Molecular endocrinology (Baltimore, Md.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 57 citations in OpenAlex.
- The Neurod1/4-Ntrk3-Src pathway regulates gonadotrope cell adhesion and motility.Cell death discovery · 2023Article
- Peptidylarginine deiminase 2 regulates expression of DGCR8 affecting miRNA biogenesis in gonadotrope cells.Reproduction (Cambridge, England) · 2023Article
- Advances in the Regulation of Mammalian Follicle-Stimulating Hormone Secretion.Animals : an open access journal from MDPI · 2021Review
- Post-Transcriptional Regulation ofInternational journal of molecular sciences · 2021Review
- Long extensions with varicosity-like structures in gonadotrope Lh cells facilitate clustering in medaka pituitary culture.PloS one · 2021Article
- Stress-mediated convergence of splicing landscapes in male and female rock doves.BMC genomics · 2020Article
- Reduction of miR-212 contributes to pituitary adenoma cell invasion via targeting c-Met.The Kaohsiung journal of medical sciences · 2020Article
- Novel Regulatory Factors in the Hypothalamic-Pituitary-Ovarian Axis of Hens at Four Developmental Stages.Frontiers in genetics · 2020Article
- GnRH-A Key Regulator of FSH.Endocrinology · 2019Review
- The Stress-Responding miR-132-3p Shows Evolutionarily Conserved Pathway Interactions.Cellular and molecular neurobiology · 2018Article
- Multifaceted Targeting of the Chromatin Mediates Gonadotropin-Releasing Hormone Effects on Gene Expression in the Gonadotrope.Frontiers in endocrinology · 2018Review
- Deep sequencing of the transcriptome in the anterior pituitary of heifers before and after ovulation.The Journal of veterinary medical science · 2017Article
- Gonadotropin releasing hormone activation of the mTORC2/Rictor complex regulates actin remodeling and ERK activity in LβT2 cells.Molecular and cellular endocrinology · 2017Article
- GnRH Induces ERK-Dependent Bleb Formation in Gonadotrope Cells, Involving Recruitment of Members of a GnRH Receptor-Associated Signalosome to the Blebs.Frontiers in endocrinology · 2017Article
- Functional Role of Gonadotrope Plasticity and Network Organization.Frontiers in endocrinology · 2017Review
- A regulatory loop between miR-132 and miR-125b involved in gonadotrope cells desensitization to GnRH.Scientific reports · 2016Article
- MicroRNA-132 promotes estradiol synthesis in ovarian granulosa cells via translational repression of Nurr1.Reproductive biology and endocrinology : RB&E · 2015Article
- Rapid communication: A microRNA-132/212 pathway mediates GnRH activation of FSH expression.Molecular endocrinology (Baltimore, Md.) · 2015Article
- Gonadotrope-specific deletion of Dicer results in severely suppressed gonadotropins and fertility defects.The Journal of biological chemistry · 2015Article
- MicroRNA in teleost fish.Genome biology and evolution · 2014Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
GnRH is central to the regulation of reproductive function. It acts on pituitary gonadotropes to stimulate LH and FSH synthesis and secretion. We had previously presented evidence for translational control of LHβ synthesis; therefore we investigated whether micro-RNAs might play a role in GnRH regulation in LβT2 cells. We show here that GnRH strongly induces the AK006051 gene transcript that encodes two micro-RNAs, miR-132 and miR-212, within the first intron. We show furthermore that the AK006051 promoter region is highly GnRH responsive. We verify that the p250Rho GTPase activating protein (GAP) is a target of miR-132/212 and show that GnRH treatment leads to a decrease in mRNA and protein expression. This reduction is blocked by an anti-miR to miR-132/212 and mimicked by a pre-miR-132. GnRH inhibits p250RhoGAP expression through a miR-132/212 response element within the 3'-untranslated region. The loss of p250RhoGAP expression leads to activation of Rac and marked increases in both the number and length of neurite-like processes extending from the cell. Knockdown of p250RhoGAP by small interfering RNA induces the same morphological changes observed with GnRH treatment. In addition, loss of p250RhoGAP causes an increase in cellular motility. Our findings suggest a novel pathway regulating long-term changes in cellular motility and process formation via the GnRH induction of miR-132/212 with the subsequent down-regulation of p250RhoGAP.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.