ArticleChromosoma2011
Atomic force microscope imaging of chromatin assembled in Xenopus laevis egg extract.
Article in Chromosoma, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- DNA-binding proteins studied by mechanical manipulation and AFM imaging of single DNA molecules.Biophysics reports · 2022Article
- Direct monitoring of the stepwise condensation of kinetoplast DNA networks.Scientific reports · 2021Article
- Oncofetal HMGA2 effectively curbs unconstrained (+) and (-) DNA supercoiling.Scientific reports · 2017Article
- Transcriptional Repressor TrmBL2 from Thermococcus kodakarensis Forms Filamentous Nucleoprotein Structures and Competes with Histones for DNA Binding in a Salt- and DNA Supercoiling-dependent Manner.The Journal of biological chemistry · 2015Article
- Mechanochemical regulations of RPA's binding to ssDNA.Scientific reports · 2015Article
- Locus of enterocyte effacement-encoded regulator (Ler) of pathogenic Escherichia coli competes off histone-like nucleoid-structuring protein (H-NS) through noncooperative DNA binding.The Journal of biological chemistry · 2014Article
- Mechanism of DNA organization by Mycobacterium tuberculosis protein Lsr2.Nucleic acids research · 2013Article
- The nucleoid-associated protein Dan organizes chromosomal DNA through rigid nucleoprotein filament formation in E. coli during anoxia.Nucleic acids research · 2013Article
- Histone H1 compacts DNA under force and during chromatin assembly.Molecular biology of the cell · 2012Article
- Gene silencing H-NS paralogue StpA forms a rigid protein filament along DNA that blocks DNA accessibility.Nucleic acids research · 2012Article
- Physical organization of DNA by multiple non-specific DNA-binding modes of integration host factor (IHF).PloS one · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Gaps persist in our understanding of chromatin lower- and higher-order structures. Xenopus egg extracts provide a way to study essential chromatin components which are difficult to manipulate in living cells, but nanoscale imaging of chromatin assembled in extracts poses a challenge. We describe a method for preparing chromatin assembled in extracts for atomic force microscopy (AFM) utilizing restriction enzyme digestion followed by transferring to a mica surface. Using this method, we find that buffer dilution of the chromatin assembly extract or incubation of chromatin in solutions of low ionic strength results in loosely compacted chromatin fibers that are prone to unraveling into naked DNA. We also describe a method for direct AFM imaging of chromatin which does not utilize restriction enzymes and reveals higher-order fibers of varying widths. Due to the capability of controlling chromatin assembly conditions, we believe these methods have broad potential for studying physiologically relevant chromatin structures.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.