ArticleGenetics2011
Insect population control by homing endonuclease-based gene drive: an evaluation in Drosophila melanogaster.
Article in Genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed.
- Review
- Advances in CRISPR gene drives for mosquito population control.Current opinion in microbiology · 2026Review
- Germline Cas9 promoters with improved performance for homing gene drive.Nature communications · 2024Article
- A small-molecule approach to restore female sterility phenotype targeted by a homing suppression gene drive in the fruit pest Drosophila suzukii.PLoS genetics · 2024Article
- Manipulating the Destiny of Wild Populations Using CRISPR.Annual review of genetics · 2023Review
- CRISPR/Cas9-based split homing gene drive targetingProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Daisy-chain gene drives: The role of low cut-rate, resistance mutations, and maternal deposition.PLoS genetics · 2022Article
- A New Approach to Develop Resistant Cultivars Against the Plant Pathogens: CRISPR Drives.Frontiers in plant science · 2022Article
- Genetic Approaches for Controlling CRISPR-based Autonomous Homing Gene Drives.Frontiers in bioengineering and biotechnology · 2022Review
- Intronic gRNAs for the Construction of Minimal Gene Drive Systems.Frontiers in bioengineering and biotechnology · 2022Article
- The Challenges in Developing Efficient and Robust Synthetic Homing Endonuclease Gene Drives.Frontiers in bioengineering and biotechnology · 2022Review
- Article
- Adequacy and sufficiency evaluation of existing EFSA guidelines for the molecular characterisation, environmental risk assessment and post-market environmental monitoring of genetically modified insects containing engineered gene drives.EFSA journal. European Food Safety Authority · 2020Article
- Chromosome drives via CRISPR-Cas9 in yeast.Nature communications · 2020Article
- A fly model establishes distinct mechanisms for synthetic CRISPR/Cas9 sex distorters.PLoS genetics · 2020Article
- Integral gene drives for population replacement.Biology open · 2019Article
- Gene drive systems: do they have a place in agricultural weed management?Pest management science · 2018Review
- Behavior of homing endonuclease gene drives targeting genes required for viability or female fertility with multiplexed guide RNAs.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Gene Drive: Evolved and Synthetic.ACS chemical biology · 2018Review
- Gene drives to fight malaria: current state and future directions.Pathogens and global health · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insects play a major role as vectors of human disease as well as causing significant agricultural losses. Harnessing the activity of customized homing endonuclease genes (HEGs) has been proposed as a method for spreading deleterious mutations through populations with a view to controlling disease vectors. Here, we demonstrate the feasibility of this method in Drosophila melanogaster, utilizing the well-characterized HEG, I-SceI. In particular, we show that high rates of homing can be achieved within spermatogonia and in the female germline. We show that homed constructs continue to exhibit HEG activity in the subsequent generation and that the ectopic homing events required for initiating the strategy occur at an acceptable rate. We conclude that the requirements for successful deployment of a HEG-based gene drive strategy can be satisfied in a model dipteran and that there is a reasonable prospect of the method working in other dipterans. In characterizing the system we measured repair outcomes at the spermatogonial, spermatocyte, and spermatid stages of spermatogenesis. We show that homologous recombination is restricted to spermatogonia and that it immediately ceases when they become primary spermatocytes, indicating that the choice of DNA repair pathway in the Drosophila testis can switch abruptly during differentiation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.