ArticleProceedings of the National Academy of Sciences of the United States of America2011
Rational development of a strategy for modifying the aggregatibility of proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 57 citations in OpenAlex.
- Revealing and Exploiting the Biochemistry ofBiochemistry · 2026Review
- Mirror-image ligand discovery enabled by single-shot fast-flow synthesis of D-proteins.Nature communications · 2024Article
- Enabling chemical protein (semi)synthesisChemical science · 2022Article
- Improved Handling of Peptide Segments Using Side Chain-Based "Helping Hand" Solubilizing Tools.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A glutamic acid-based traceless linker to address challenging chemical protein syntheses.Organic & biomolecular chemistry · 2021Article
- A straightforward methodology to overcome solubility challenges for N-terminal cysteinyl peptide segments used in native chemical ligation.Chemical science · 2021Article
- Chemical biology of glycoproteins: From chemical synthesis to biological impact.Methods in enzymology · 2019Article
- Total Chemical Synthesis of Human Thyroid-Stimulating Hormone (hTSH)Tetrahedron · 2018Article
- Chemical synthesis of membrane proteins by the removable backbone modification method.Nature protocols · 2017Article
- Aligator: A computational tool for optimizing total chemical synthesis of large proteins.Bioorganic & medicinal chemistry · 2017Review
- Effect of position-specific single-point mutations and biophysical characterization of amyloidogenic peptide fragments identified from lattice corneal dystrophy patients.The Biochemical journal · 2017Article
- A Helping Hand to Overcome Solubility Challenges in Chemical Protein Synthesis.Journal of the American Chemical Society · 2016Article
- The winding pathway to erythropoietin along the chemistry-biology frontier: a success at last.Angewandte Chemie (International ed. in English) · 2013Review
- Use of a small peptide fragment as an inhibitor of insulin fibrillation process: a study by high and low resolution spectroscopy.PloS one · 2013Article
- At last: erythropoietin as a single glycoform.Angewandte Chemie (International ed. in English) · 2012Article
- An advance in the chemical synthesis of homogeneous N-linked glycopolypeptides by convergent aspartylation.Angewandte Chemie (International ed. in English) · 2012Article
- Engineering of therapeutic polypeptides through chemical synthesis: early lessons from human parathyroid hormone and analogues.Journal of the American Chemical Society · 2012Article
- Toward Homogeneous Erythropoietin: Application of Metal Free Dethiylation in the Chemical Synthesis of the Ala79-Arg166 Glycopeptide Domain.Israel journal of chemistry · 2011Article
- An advance in proline ligation.Journal of the American Chemical Society · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The conversion of peptide and proteins from their soluble state into well-organized aggregates, together with the accompanied oxidation of methionine residue, presents a significant challenge to human health, to the manufacture of protein therapeutics, and to the synthesis of proteins and glycoproteins. Despite their fundamental importance, little is known about the molecular basis of these two side reactions and their control. Here, using chemical peptide synthesis, we further confirmed the importance of the balance between hydrophobic interactions and electrostatic repulsive forces in inducing and inhibiting aggregation and methionine oxidation. Most importantly, through extending the established principle, we are able to effectively stabilize the problematic peptide fragment through the attachment of cleavable arginine tags. Future applications of our approach are expected to facilitate the synthesis and study of difficult peptides, proteins, and glycoproteins and will provide more opportunities for the optimization of protein biopharmaceuticals and for the development of cell-permeable biomolecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.