ArticleProceedings of the National Academy of Sciences of the United States of America2011
Vertical nanopillars for highly localized fluorescence imaging.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Vertically Aligned Nanopillar Electrodes: Engineered Interfaces for Electrophysiology and Cell-Electrode Coupling.Small methods · 2026Review
- Plasmonic Nanopillars-A Brief Investigation of Fabrication Techniques and Biological Applications.Biosensors · 2023Review
- Semi-Implantable Bioelectronics.Nano-micro letters · 2022Review
- New perspectives on the roles of nanoscale surface topography in modulating intracellular signaling.Current opinion in solid state & materials science · 2021Article
- High-Aspect-Ratio Nanostructured Surfaces as Biological Metamaterials.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- Spatioselective functionalization of gold nanopillar arrays.Nanoscale advances · 2019Article
- Interfacing Cells with Vertical Nanoscale Devices: Applications and Characterization.Annual review of analytical chemistry (Palo Alto, Calif.) · 2018Review
- The Role of Membrane Curvature in Nanoscale Topography-Induced Intracellular Signaling.Accounts of chemical research · 2018Review
- Scalable Fabrication Framework of Implantable Ultrathin and Flexible Probes with Biodegradable Sacrificial Layers.Nano letters · 2017Article
- Article
- Impact of degradable nanowires on long-term brain tissue responses.Journal of nanobiotechnology · 2016Article
- Nanotechnology and regenerative therapeutics in plastic surgery: The next frontier.Journal of plastic, reconstructive & aesthetic surgery : JPRAS · 2016Review
- Sub-10 nm near-field localization by plasmonic metal nanoaperture arrays with ultrashort light pulses.Scientific reports · 2015Article
- Interfacing Inorganic Nanowire Arrays and Living Cells for Cellular Function Analysis.Small (Weinheim an der Bergstrasse, Germany) · 2015Review
- Localization-based full-field microscopy: how to attain super-resolved images.Scientific reports · 2015Article
- Vertical nanopillars for in situ probing of nuclear mechanics in adherent cells.Nature nanotechnology · 2015Article
- Metal Organic Framework Micro/Nanopillars of Cu(BTC)·3H₂O and Zn(ADC)·DMSO.Nanomaterials (Basel, Switzerland) · 2015Article
- ZnO nanorod array grown on Ag layer: a highly efficient fluorescence enhancement platform.Scientific reports · 2015Article
- Quantification of nanowire penetration into living cells.Nature communications · 2014Article
- Synthetic nanoelectronic probes for biological cells and tissues.Annual review of analytical chemistry (Palo Alto, Calif.) · 2013Review
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4 authors.
Funding
Abstract
Observing individual molecules in a complex environment by fluorescence microscopy is becoming increasingly important in biological and medical research, for which critical reduction of observation volume is required. Here, we demonstrate the use of vertically aligned silicon dioxide nanopillars to achieve below-the-diffraction-limit observation volume in vitro and inside live cells. With a diameter much smaller than the wavelength of visible light, a transparent silicon dioxide nanopillar embedded in a nontransparent substrate restricts the propagation of light and affords evanescence wave excitation along its vertical surface. This effect creates highly confined illumination volume that selectively excites fluorescence molecules in the vicinity of the nanopillar. We show that this nanopillar illumination can be used for in vitro single-molecule detection at high fluorophore concentrations. In addition, we demonstrate that vertical nanopillars interface tightly with live cells and function as highly localized light sources inside the cell. Furthermore, specific chemical modification of the nanopillar surface makes it possible to locally recruit proteins of interest and simultaneously observe their behavior within the complex, crowded environment of the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.