ArticleBMC research notes2011
Novel sulI binary vectors enable an inexpensive foliar selection method in Arabidopsis.
Article in BMC research notes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Development of an improved construct for spectinomycin selection in plant transformation.BMC research notes · 2025Article
- Two ARGONAUTE proteins loaded with transposon-derived small RNAs are associated with the reproductive cell lineage in Arabidopsis.The Plant cell · 2024Article
- Salicylic acid and RNA interference mediate antiviral immunity of plant stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Sulfadiazine and phosphinothricin selection systems optimised for the transformation of tobacco BY-2 cells.Plant cell reports · 2023Article
- Cytosolic phosphoglucose isomerase is essential for microsporogenesis and embryogenesis in Arabidopsis.Plant physiology · 2023Article
- Microparticle-mediated CRISPR DNA delivery for genome editing in poplar.Frontiers in plant science · 2023Article
- Phenotypic Examination ofPlants (Basel, Switzerland) · 2020Article
- Combined computational and experimental studies on cysteine-sulfadiazine adduct formation.Turkish journal of chemistry · 2020Article
- Establishment of CRISPR/Cas9 Genome Editing in Witloof (Frontiers in genome editing · 2020Article
- Transgene stacking in potato using the GAANTRY system.BMC research notes · 2019Article
- Xyloglucan exoglycosidases in the monocot model Brachypodium distachyon and the conservation of xyloglucan disassembly in angiosperms.Plant molecular biology · 2019Article
- A highly efficient sulfadiazine selection system for the generation of transgenic plants and algae.Plant biotechnology journal · 2019Article
- Soluble and Membrane-Bound β-Glucosidases Are Involved in Trimming the Xyloglucan Backbone.Plant physiology · 2017Article
- Identification of Arabidopsis GPAT9 (At5g60620) as an Essential Gene Involved in Triacylglycerol Biosynthesis.Plant physiology · 2016Article
- Screening promoters for Anthurium transformation using transient expression.Plant cell reports · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSulfonamide resistance is conferred by the sulI gene found on many Enterobacteriaceae R plasmids and Tn21 type transposons. The sulI gene encodes a sulfonamide insensitive dihydropteroate synthase enzyme required for folate biosynthesis. Transformation of tobacco, potato or Arabidopsis using sulI as a selectable marker generates sulfadiazine-resistant plants. Typically sulI-based selection of transgenic plants is performed on tissue culture media under sterile conditions.
findingsA set of novel binary vectors containing a sulI selectable marker expression cassette were constructed and used to generate transgenic Arabidopsis. We demonstrate that the sulI selectable marker can be utilized for direct selection of plants grown in soil with a simple foliar spray application procedure. A highly effective and inexpensive high throughput screening strategy to identify transgenic Arabidopsis without use of tissue culture was developed.
conclusionNovel sulI-containing Agrobacterium binary vectors designed to over-express a gene of interest or to characterize a test promoter in transgenic plants have been constructed. These new vector tools combined with the various beneficial attributes of sulfonamide selection and the simple foliar screening strategy provide an advantageous alternative for plant biotechnology researchers. The set of binary vectors is freely available upon request.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.