ArticleThe New phytologist2011
An experimental method to facilitate the identification of hybrid sporophytes in the moss Physcomitrella patens using fluorescent tagged lines.
Article in The New phytologist, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Moss BRCA2 lacking the canonical DNA-binding domain promotes homologous recombination and binds to DNA.Nucleic acids research · 2025Article
- Protocol: an improved method for inducing sporophyte generation in the model moss Physcomitrium patens under nitrogen starvation.Plant methods · 2023Article
- Article
- Physcomitrium patens Protoplasting and Protoplast Transfection.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens inFrontiers in plant science · 2022Article
- The nuclear GUCT domain-containing DEAD-box RNA helicases govern gametophytic and sporophytic development in Physcomitrium patens.Plant molecular biology · 2021Article
- Characterisation of evolutionarily conserved key players affecting eukaryotic flagellar motility and fertility using a moss model.The New phytologist · 2020Article
- Functional expression and characterization of cinnamic acid 4-hydroxylase from the hornwort Anthoceros agrestis in Physcomitrella patens.Plant cell reports · 2020Article
- The MossThe Plant cell · 2020Review
- Single Nucleotide Polymorphism Charting ofFrontiers in plant science · 2020Article
- Stomata and Sporophytes of the Model MossFrontiers in plant science · 2020Article
- Conditional genetic screen in Physcomitrella patens reveals a novel microtubule depolymerizing-end-tracking protein.PLoS genetics · 2018Article
- Moss genetics and the way forward.PLoS genetics · 2018Article
- Genetic Regulation of the 2D to 3D Growth Transition in the Moss Physcomitrella patens.Current biology : CB · 2018Article
- GLUTAMATE RECEPTOR-LIKE channels are essential for chemotaxis and reproduction in mosses.Nature · 2017Article
- Non-model model organisms.BMC biology · 2017Article
- Selfing in Haploid Plants and Efficacy of Selection: Codon Usage Bias in the Model Moss Physcomitrella patens.Genome biology and evolution · 2017Article
- Combination of the EndogenousFrontiers in plant science · 2017Article
- Microscopy ofPlant methods · 2017Article
- Sporophyte Formation and Life Cycle Completion in Moss Requires Heterotrimeric G-Proteins.Plant physiology · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
• The sequencing of the Physcomitrella patens genome, combined with the high frequency of gene targeting in this species, makes it ideal for reverse genetic studies. For forward genetic studies, experimental crosses and genetic analysis of progeny are essential. • Since P. patens is monoicous, producing both male and female gametes on the same gametophore, and undergoing self-fertilization at a high frequency, the identification of crossed sporophytes is difficult. Usually spores from many sporophytes from a mixed culture must be tested for the production of recombinant progeny. • Here, we describe the use of transgenic lines that express a fluorescent transgene constitutively, to provide a direct visual screen for hybrid sporophytes. • We show that segregations in crosses obtained with this technique are as expected, and demonstrate its utility for the study of the rate of outcrossing between three isolates of P. patens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.