ArticleProteomics2011
Mining the soluble chloroplast proteome by affinity chromatography.
Article in Proteomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- A voyage of reprogrammable metabolic bioengineering reshapes plant defense: from editing tools to synthetic systems.Frontiers in plant science · 2026Review
- Comparison of plastid proteomes points towards a higher plastidial redox turnover in vascular tissues than in mesophyll cells.Journal of experimental botany · 2023Article
- Plant deubiquitinases: from structure and activity to biological functions.Plant cell reports · 2023Review
- The intermediate in a nitrate-responsive ω-amidase pathway in plants may signal ammonium assimilation status.Plant physiology · 2023Article
- The tomato chloroplast stromal proteome compendium elucidated by leveraging a plastid protein-localization prediction Atlas.Frontiers in plant science · 2023Article
- The Arabidopsis Protein CGL20 Is Required for Plastid 50S Ribosome Biogenesis.Plant physiology · 2020Article
- Article
- Barley cysteine protease PAP14 plays a role in degradation of chloroplast proteins.Journal of experimental botany · 2019Article
- Dissection of Phenotypic and Genetic Variation of Drought-Related Traits in Diverse Chinese Wheat Landraces.The plant genome · 2019Article
- Application of Proteomics Technologies in Oil Palm Research.The protein journal · 2018Review
- The Starch Granule-Associated Protein EARLY STARVATION1 Is Required for the Control of Starch Degradation in Arabidopsis thaliana Leaves.The Plant cell · 2016Article
- The conserved endoribonuclease YbeY is required for chloroplast ribosomal RNA processing in Arabidopsis.Plant physiology · 2015Article
- Pre-fractionation strategies to resolve pea (Pisum sativum) sub-proteomes.Frontiers in plant science · 2015Review
- Higher plant proteins of cyanobacterial origin: are they or are they not preferentially targeted to chloroplasts?Molecular plant · 2014Article
- Identification of CP12 as a Novel Calcium-Binding Protein in Chloroplasts.Plants (Basel, Switzerland) · 2013Article
- Defining the core proteome of the chloroplast envelope membranes.Frontiers in plant science · 2013Article
- Review
- The core of chloroplast nucleoids contains architectural SWIB domain proteins.The Plant cell · 2012Article
- Chloroplast-localized protein kinases: a step forward towards a complete inventory.Journal of experimental botany · 2012Article
- Plant organellar calcium signalling: an emerging field.Journal of experimental botany · 2012Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplasts are fundamental organelles enabling plant photoautotrophy. Besides their outstanding physiological role in fixation of atmospheric CO(2), they harbor many important metabolic processes such as biosynthesis of amino acids, vitamins or hormones. Technical advances in MS allowed the recent identification of most chloroplast proteins. However, for a deeper understanding of chloroplast function it is important to obtain a complete list of constituents, which is so far limited by the detection of low-abundant proteins. Therefore, we developed a two-step strategy for the enrichment of low-abundant soluble chloroplast proteins from Pisum sativum and their subsequent identification by MS. First, chloroplast protein extracts were depleted from the most abundant protein ribulose-1,5-bisphosphate carboxylase/oxygenase by SEC or heating. Further purification was carried out by affinity chromatography, using ligands specific for ATP- or metal-binding proteins. By these means, we were able to identify a total of 448 proteins including 43 putative novel chloroplast proteins. Additionally, the chloroplast localization of 13 selected proteins was confirmed using yellow fluorescent protein fusion analyses. The selected proteins included a phosphoglycerate mutase, a cysteine protease, a putative protein kinase and an EF-hand containing substrate carrier protein, which are expected to exhibit important metabolic or regulatory functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.