ArticlePhotosynthesis research2011
Localization of putative carbonic anhydrases in two marine diatoms, Phaeodactylum tricornutum and Thalassiosira pseudonana.
Article in Photosynthesis research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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55 citing papers in PubMed, 149 citations in OpenAlex.
- NPF2 is involved in intracellular pH regulation and ion balance in the diatom Phaeodactylum tricornutum.The New phytologist · 2026Article
- Comprehensive identification and subcellular localization prediction of carbonic anhydrases in the marine haptophyte Tisochrysis lutea based on a refined genome annotation.Archives of microbiology · 2026Article
- Genome-wide identification and expression analysis of theFrontiers in plant science · 2026Article
- ASAFind 2.0: multi-class protein targeting prediction for diatoms and algae with complex plastids.The Plant journal : for cell and molecular biology · 2025Article
- SAGA1 and MITH1 produce matrix-traversing membranes in the CONature plants · 2024Article
- Low-CO2-inducible bestrophins outside the pyrenoid sustain high photosynthetic efficacy in diatoms.Plant physiology · 2024Article
- A linker protein from a red-type pyrenoid phase separates with Rubisco via oligomerizing sticker motifs.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Localization and characterization θ carbonic anhydrases in Thalassiosira pseudonana.Photosynthesis research · 2023Article
- α-CAs from Photosynthetic Organisms.International journal of molecular sciences · 2022Review
- Adaptive responses of marine diatoms to zinc scarcity and ecological implications.Nature communications · 2022Article
- Phytate as a Phosphorus Nutrient with Impacts on Iron Stress-Related Gene Expression for Phytoplankton: Insights from the DiatomApplied and environmental microbiology · 2022Article
- The differential ability of two species of seagrass to use carbon dioxide and bicarbonate and their modelled response to rising concentrations of inorganic carbon.Frontiers in plant science · 2022Article
- Biogeochemical feedbacks to ocean acidification in a cohesive photosynthetic sediment.Scientific reports · 2021Article
- Diploid genomic architecture of Nitzschia inconspicua, an elite biomass production diatom.Scientific reports · 2021Article
- The Role of Extracellular Carbonic Anhydrase in Biogeochemical Cycling: Recent Advances and Climate Change Responses.International journal of molecular sciences · 2021Review
- Characterization of a COMarine biotechnology (New York, N.Y.) · 2021Article
- Review
- Review
- Insights on the Functions and Ecophysiological Relevance of the Diverse Carbonic Anhydrases in Microalgae.International journal of molecular sciences · 2020Review
- Regulation of Carbon Metabolism by Environmental Conditions: A Perspective From Diatoms and Other Chromalveolates.Frontiers in plant science · 2020Review
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is believed that intracellular carbonic anhydrases (CAs) are essential components of carbon concentrating mechanisms in microalgae. In this study, putative CA-encoding genes were identified in the genome sequences of the marine diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana. Subsequently, the subcellular localizations of the encoded proteins were determined. Nine and thirteen CA sequences were found in the genomes of P. tricornutum and T. pseudonana, respectively. Two of the β-CA genes in P. tricornutum corresponded to ptca1 and ptca2 identified previously. Immunostaining transmission electron microscopy of a PtCA1:YFP fusion expressed in the cells of P. tricornutum clearly showed the localization of PtCA1 within the central part of the pyrenoid structure in the chloroplast. Besides these two β-CA genes, P. tricornutum likely contains five α- and two γ-CA genes, whereas T. pseudonana has three α-, five γ-, four δ-, and one ζ-CA genes. Semi-quantitative reverse transcription PCR performed on mRNA from the two diatoms grown in changing light and CO(2) conditions revealed that levels of six putative α- and γ-CA mRNAs in P. tricornutum did not change between cells grown in air-level CO(2) and 5% CO(2). However, mRNA levels of one putative α-CA gene, CA-VII in P. tricornutum, were reduced in the dark compared to that in the light. In T. pseudonana, mRNA accumulation levels of putative α-CA (CA-1), ζ-CA (CA-3) and δ-CA (CA-7) were analyzed and all levels found to be significantly reduced when cells were grown in 0.16% CO(2). Intercellular localizations of eight putative CAs were analyzed by expressing GFP fusion in P. tricornutum and T. pseudonana. In P. tricornutum, CA-I and II localized in the periplastidial compartment, CA-III, VI, VII were found in the chloroplast endoplasmic reticulum, and CA-VIII was localized in the mitochondria. On the other hand, T. pseudonana CA-1 localized in the stroma and CA-3 was found in the periplasm. These results suggest that CAs are constitutively present in the four chloroplastic membrane systems in P. tricornutum and that CO(2) responsive CAs occur in the pyrenoid of P. tricornutum, and in the stroma and periplasm of T. pseudonana.
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