ArticleMolecular vision2011
Establishment of a continuous untransfected human corneal endothelial cell line and its biocompatibility to denuded amniotic membrane.
Article in Molecular vision, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Research Progress of Polymer Biomaterials as Scaffolds for Corneal Endothelium Tissue Engineering.Nanomaterials (Basel, Switzerland) · 2023Review
- Nanomolar Potency Aminophenyltriazine CFTR Activator Reverses Corneal Epithelial Injury in a Mouse Model of Dry Eye.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2020Article
- Bioenergetic Crosstalk between Mesenchymal Stem Cells and various Ocular Cells through the intercellular trafficking of Mitochondria.Theranostics · 2020Article
- Dose- and Time-Dependent Cytotoxicity of Carteolol in Corneal Endothelial Cells and the Underlying Mechanisms.Frontiers in pharmacology · 2020Article
- Apoptotic effects of norfloxacin on corneal endothelial cells.Naunyn-Schmiedeberg's archives of pharmacology · 2020Article
- Article
- Sodium Ferulate Attenuates Lidocaine-Induced Corneal Endothelial Impairment.Oxidative medicine and cellular longevity · 2018Article
- Cytotoxicity of atropine to human corneal endothelial cells by inducing mitochondrion-dependent apoptosis.Experimental biology and medicine (Maywood, N.J.) · 2016Article
- Transcriptomic Analysis of Cultured Corneal Endothelial Cells as a Validation for Their Use in Cell Replacement Therapy.Cell transplantation · 2016Article
- Substrates for Expansion of Corneal Endothelial Cells towards Bioengineering of Human Corneal Endothelium.Journal of functional biomaterials · 2015Review
- Cytotoxic effects of betaxolol on healthy corneal endothelial cells both in vitro and in vivo.International journal of ophthalmology · 2014Article
- Transforming growth factor-β2 induces morphological alteration of human corneal endothelial cells in vitro.International journal of ophthalmology · 2014Article
- Transplantation of tissue-engineered human corneal endothelium in cat models.Molecular vision · 2013Article
- Establishment of functioning human corneal endothelial cell line with high growth potential.PloS one · 2012Article
- Establishment of an untransfected human corneal stromal cell line and its biocompatibility to acellular porcine corneal stroma.International journal of ophthalmology · 2012Article
- In vitro reconstruction and characterization of tissue-engineered human corneal epithelium with seeder cells from an untransfected human corneal epithelial cell line.International journal of ophthalmology · 2012Article
- Establishment of an untransfected human corneal epithelial cell line and its biocompatibility with denuded amniotic membrane.International journal of ophthalmology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo establish an untransfected human corneal endothelial (HCE) cell line and characterize its biocompatibility to denuded amniotic membrane (dAM).
methodsPrimary culture was initiated with a pure population of HCE cells in DMEM/F12 media (pH 7.2) containing 20% fetal bovine serum and various supplements. The established cell line was characterized by growth property, chromosome analysis, morphology recovery, tumorigenicity assay, and expression of marker proteins, cell-junction proteins, and membrane transport proteins. The biocompatibility of HCE cells to dAM was evaluated by light microscopy, alizarin red staining, immunofluorescence assay, and electron microscopy.
resultsHCE cells proliferated to confluence 6 weeks later in primary culture and have been subcultured to passage 224 so far. A continuous untransfected HCE cell line with a population doubling time of 26.20 h at passage 101 has been established. Results of chromosome analysis, morphology, combined with the results of expression of marker protein, cell-junction protein and membrane transport protein, suggested that the cells retained HCE cell properties and potencies to form cell junctions and perform membrane transport. Furthermore, HCE cells, without any tumorigenicity, could form confluent cell sheets on dAMs. The single layer sheets that attached tightly to dAMs had similar morphology and structure to those of HCE in situ and had an average cell density of 3,413±111 cells/mm².
conclusionsAn untransfected and non-tumorigenic HCE cell line has been established, and the cells maintained positive expression of marker proteins, cell-junction proteins and membrane transport proteins. The cell line, with excellent biocompatibility to dAM, might be used for in vitro reconstruction of HCE and provides a promising method for the treatment of diseases caused by corneal endothelial disorders.
Indexed as
Identifiers
21365020PMC3042360W2131969667What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.