ArticlePloS one2011
Genome-wide mapping of DNA strand breaks.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Causes and consequences of DNA double-stranded breaks in cardiovascular disease.Molecular and cellular biochemistry · 2025Review
- DNA Double Strand Break and Response Fluorescent Assays: Choices and Interpretation.International journal of molecular sciences · 2024Review
- The increase in cell death rates in caloric restricted cells of the yeast helicase mutant rrm3 is Sir complex dependent.Scientific reports · 2023Article
- TdT-dUTP DSB End Labeling (TUDEL), for Specific, Direct In Situ Labeling of DNA Double Strand Breaks.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- High-resolution, ultrasensitive and quantitative DNA double-strand break labeling in eukaryotic cells using i-BLESS.Nature protocols · 2021Article
- Genome-wide detection of DNA double-strand breaks by in-suspension BLISS.Nature protocols · 2020Article
- Genome-wide Nucleotide-Resolution Mapping of DNA Replication Patterns, Single-Strand Breaks, and Lesions by GLOE-Seq.Molecular cell · 2020Article
- Emerging Technologies for Genome-Wide Profiling of DNA Breakage.Frontiers in genetics · 2020Review
- CRISPR/CAS9 targeted CAPTURE of mammalian genomic regions for characterization by NGS.Scientific reports · 2019Article
- Motor neuron disease-associated loss of nuclear TDP-43 is linked to DNA double-strand break repair defects.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Review
- Binding of Multiple Rap1 Proteins Stimulates Chromosome Breakage Induction during DNA Replication.PLoS genetics · 2015Article
- Quantifying DNA double-strand breaks induced by site-specific endonucleases in living cells by ligation-mediated purification.Nature protocols · 2014Article
- Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing.Nature methods · 2013Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Determination of cellular DNA damage has so far been limited to global assessment of genome integrity whereas nucleotide-level mapping has been restricted to specific loci by the use of specific primers. Therefore, only limited DNA sequences can be studied and novel regions of genomic instability can hardly be discovered. Using a well-characterized yeast model, we describe a straightforward strategy to map genome-wide DNA strand breaks without compromising nucleotide-level resolution. This technique, termed "damaged DNA immunoprecipitation" (dDIP), uses immunoprecipitation and the terminal deoxynucleotidyl transferase-mediated dUTP-biotin end-labeling (TUNEL) to capture DNA at break sites. When used in combination with microarray or next-generation sequencing technologies, dDIP will allow researchers to map genome-wide DNA strand breaks as well as other types of DNA damage and to establish a clear profiling of altered genes and/or intergenic sequences in various experimental conditions. This mapping technique could find several applications for instance in the study of aging, genotoxic drug screening, cancer, meiosis, radiation and oxidative DNA damage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.