ArticlePloS one2011
Microdroplet-enabled highly parallel co-cultivation of microbial communities.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
74 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Production of Gamma-Aminobutyric Acid from Lactic Acid Bacteria: A Systematic Review.International journal of molecular sciences · 2020Pooled it
- Microdroplet-based high-throughput screening for antagonistic bacteria targeting penaeid shrimp pathogenic Vibrio harveyi.Scientific reports · 2026Article
- Harnessing droplet microfluidics and morphology-based deep learning for the label-free study of polymicrobial-phage interactions.Communications biology · 2025Article
- Cellulose-functionalized magnetic nanoparticle-mediated isolation of novel cellulolytic bacteria.Applied and environmental microbiology · 2025Article
- A digital plating platform for robust and versatile microbial detection and analysis.Scientific reports · 2025Article
- Habitat fragmentation enhances microbial collective defence.Journal of the Royal Society, Interface · 2025Article
- Microbial community interactions on a chip.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Fragmented micro-growth habitats present opportunities for alternative competitive outcomes.Nature communications · 2024Article
- Contributions of Gamma-Aminobutyric Acid (GABA) Produced by Lactic Acid Bacteria on Food Quality and Human Health: Current Applications and Future Prospects.Foods (Basel, Switzerland) · 2024Review
- Serial cultures in invert emulsion and monophase systems for microbial community shaping and propagation.Microbial cell factories · 2024Article
- Learning beyond-pairwise interactions enables the bottom-up prediction of microbial community structure.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Microbiome metabolite quantification methods enabling insights into human health and disease.Methods (San Diego, Calif.) · 2024Review
- Research progress in isolation and identification of rumen probiotics.Frontiers in cellular and infection microbiology · 2024Review
- Double emulsions as a high-throughput enrichment and isolation platform for slower-growing microbes.ISME communications · 2023Article
- [Application of Droplet-Based Microfluidics in Microbial Research].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2023Review
- Microfluidics: Insights into Intestinal Microorganisms.Microorganisms · 2023Review
- Film-Shaped Self-Powered Electro-Osmotic Micropump Array.Micromachines · 2023Article
- Invert emulsions alleviate biotic interactions in bacterial mixed culture.Microbial cell factories · 2023Article
- Genomic insights into versatile lifestyle of three new bacterial candidate phyla.Science China. Life sciences · 2022Article
- From Microtiter Plates to Droplets-There and Back Again.Micromachines · 2022Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Microbial interactions in natural microbiota are, in many cases, crucial for the sustenance of the communities, but the precise nature of these interactions remain largely unknown because of the inherent complexity and difficulties in laboratory cultivation. Conventional pure culture-oriented cultivation does not account for these interactions mediated by small molecules, which severely limits its utility in cultivating and studying "unculturable" microorganisms from synergistic communities. In this study, we developed a simple microfluidic device for highly parallel co-cultivation of symbiotic microbial communities and demonstrated its effectiveness in discovering synergistic interactions among microbes. Using aqueous micro-droplets dispersed in a continuous oil phase, the device could readily encapsulate and co-cultivate subsets of a community. A large number of droplets, up to ∼1,400 in a 10 mm × 5 mm chamber, were generated with a frequency of 500 droplets/sec. A synthetic model system consisting of cross-feeding E. coli mutants was used to mimic compositions of symbionts and other microbes in natural microbial communities. Our device was able to detect a pair-wise symbiotic relationship when one partner accounted for as low as 1% of the total population or each symbiont was about 3% of the artificial community.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.