ArticleMolecular systems biology2011
A synthetic library of RNA control modules for predictable tuning of gene expression in yeast.
Article in Molecular systems biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 82 citations in OpenAlex.
- Selection of DNA Aptamers Against Parathyroid Hormone for Electrochemical Impedimetric Biosensor System Development.Biotechnology and applied biochemistry · 2025Article
- Combinatorial optimization of gene expression through recombinase-mediated promoter and terminator shuffling in yeast.Nature communications · 2024Article
- Optogenetic spatial patterning of cooperation in yeast populations.Nature communications · 2024Article
- RNAi-based Boolean gates in the yeastFrontiers in bioengineering and biotechnology · 2024Article
- Aptamers, Riboswitches, and Ribozymes inLife (Basel, Switzerland) · 2021Review
- Design of RNA hairpin modules that predictably tune translation in yeast.Synthetic biology (Oxford, England) · 2018Article
- Strategies for manipulation of oxygen utilization by the electron transfer chain in microbes for metabolic engineering purposes.Journal of industrial microbiology & biotechnology · 2017Review
- A genetic screen for increasing metabolic flux in the isoprenoid pathway ofMetabolic engineering communications · 2016Article
- Enhancement of protein production via the strong DIT1 terminator and two RNA-binding proteins in Saccharomyces cerevisiae.Scientific reports · 2016Article
- Synthetic Transcription Amplifier System for Orthogonal Control of Gene Expression in Saccharomyces cerevisiae.PloS one · 2016Article
- Analytics for Metabolic Engineering.Frontiers in bioengineering and biotechnology · 2015Review
- Modular, multi-input transcriptional logic gating with orthogonal LacI/GalR family chimeras.ACS synthetic biology · 2014Article
- Molecular tools for chemical biotechnology.Current opinion in biotechnology · 2013Review
- Spanning high-dimensional expression space using ribosome-binding site combinatorics.Nucleic acids research · 2013Article
- Synthetic biology: advancing the design of diverse genetic systems.Annual review of chemical and biomolecular engineering · 2013Review
- Identification and treatment of heme depletion attributed to overexpression of a lineage of evolved P450 monooxygenases.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity.Nucleic acids research · 2012Article
- Fine-tuning gene networks using simple sequence repeats.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Ergosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes.Molecular biology of the cell · 2012Article
- Parts plus pipes: synthetic biology approaches to metabolic engineering.Metabolic engineering · 2012Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advances in synthetic biology have resulted in the development of genetic tools that support the design of complex biological systems encoding desired functions. The majority of efforts have focused on the development of regulatory tools in bacteria, whereas fewer tools exist for the tuning of expression levels in eukaryotic organisms. Here, we describe a novel class of RNA-based control modules that provide predictable tuning of expression levels in the yeast Saccharomyces cerevisiae. A library of synthetic control modules that act through posttranscriptional RNase cleavage mechanisms was generated through an in vivo screen, in which structural engineering methods were applied to enhance the insulation and modularity of the resulting components. This new class of control elements can be combined with any promoter to support titration of regulatory strategies encoded in transcriptional regulators and thus more sophisticated control schemes. We applied these synthetic controllers to the systematic titration of flux through the ergosterol biosynthesis pathway, providing insight into endogenous control strategies and highlighting the utility of this control module library for manipulating and probing biological systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.