ArticleMolecular microbiology2011
In vivo phosphorylation patterns of key stressosome proteins define a second feedback loop that limits activation of Bacillus subtilis σB.
Article in Molecular microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Phosphorylation State Dictates Bacterial Stressosome Assembly and Function.Research square · 2025Article
- Stressosome-independent but RsbT-dependent environmental stress sensing in Bacillus subtilis.Nature communications · 2025Article
- TheAccess microbiology · 2023Article
- Article
- Molecular insights into intra-complex signal transmission during stressosome activation.Communications biology · 2022Article
- The Vibrio vulnificus stressosome is an oxygen-sensor involved in regulating iron metabolism.Communications biology · 2022Article
- Phylogenetic and Phenotypic Analyses of a Collection of Food and Clinical Listeria monocytogenes Isolates Reveal Loss of Function of Sigma B from Several Clonal Complexes.Applied and environmental microbiology · 2022Article
- Acid stress signals are integrated into the σB-dependent general stress response pathway via the stressosome in the food-borne pathogen Listeria monocytogenes.PLoS pathogens · 2022Article
- Listeria monocytogenes Requires the RsbX Protein To Prevent SigB Activation under Nonstressed Conditions.Journal of bacteriology · 2022Article
- Activation of the Listeria monocytogenes Stressosome in the Intracellular Eukaryotic Environment.Applied and environmental microbiology · 2021Article
- Mutant Strains of Escherichia coli and Methicillin-Resistant Staphylococcus aureus Obtained by Laboratory Selection To Survive on Metallic Copper Surfaces.Applied and environmental microbiology · 2020Article
- Impact of osmotic stress on the phosphorylation and subcellular location of Listeria monocytogenes stressosome proteins.Scientific reports · 2020Article
- Mild Stress Conditions during Laboratory Culture Promote the Proliferation of Mutations That Negatively Affect Sigma B Activity in Listeria monocytogenes.Journal of bacteriology · 2020Article
- The σFrontiers in microbiology · 2020Review
- The Stress-Responsive Alternative Sigma Factor SigB ofFrontiers in microbiology · 2020Review
- The cryo-electron microscopy supramolecular structure of the bacterial stressosome unveils its mechanism of activation.Nature communications · 2019Article
- Use of a microfluidic platform to uncover basic features of energy and environmental stress responses in individual cells of Bacillus subtilis.PLoS genetics · 2017Article
- Mechanism and Role of Globin-Coupled Sensor Signalling.Advances in microbial physiology · 2017Review
- An O2-sensing stressosome from a Gram-negative bacterium.Nature communications · 2016Article
- To Modulate Survival under Secondary Stress Conditions, Listeria monocytogenes 10403S Employs RsbX To Downregulate σB Activity in the Poststress Recovery Stage or Stationary Phase.Applied and environmental microbiology · 2016Article
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6 authors.
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Abstract
The Bacillus subtilis stressosome is a 1.8 MDa complex that orchestrates activation of the σ(B) transcription factor by environmental stress. The complex comprises members of the RsbR co-antagonist family and the RsbS antagonist, which together form an icosahedral core that sequesters the RsbT serine-threonine kinase. Phosphorylation of this core by RsbT is associated with RsbT release, which activates downstream signalling. RsbRA, the prototype co-antagonist, is phosphorylated on T171 and T205 in vitro. In unstressed cells T171 is already phosphorylated; this is a prerequisite but not the trigger for activation, which correlates with stress-induced phosphorylation of RsbS on S59. In contrast, phosphorylation of RsbRA T205 has not been detected in vivo. Here we find (i) RsbRA is additionally phosphorylated on T205 following strong stresses, (ii) this modification requires RsbT, and (iii) the phosphorylation-deficient T205A substitution greatly increases post-stress activation of σ(B) . We infer that T205 phosphorylation constitutes a second feedback mechanism to limit σ(B) activation, operating in addition to the RsbX feedback phosphatase. Loss of RsbX function increases the fraction of phosphorylated RsbS and doubly phosphorylated RsbRA in unstressed cells. We propose that RsbX both maintains the ready state of the stressosome prior to stress and restores it post-stress.
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