ArticleJournal of structural and functional genomics2011
SAD phasing using iodide ions in a high-throughput structural genomics environment.
Article in Journal of structural and functional genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
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- A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis.Communications biology · 2023Article
- Properties and biotechnological applications of microbial deacetylase.Applied microbiology and biotechnology · 2023Review
- Structural and biochemical characterization ofProcess biochemistry (Barking, London, England) · 2023Article
- Extending the novel |ρ|-based phasing algorithm to the solution of anomalous scattering substructures from SAD data of protein crystals.Acta crystallographica. Section A, Foundations and advances · 2022Article
- Predicting drug targets by homology modelling of Pseudomonas aeruginosa proteins of unknown function.PloS one · 2021Article
- The copper(II)-binding tripeptide GHK, a valuable crystallization and phasing tag for macromolecular crystallography.Acta crystallographica. Section D, Structural biology · 2020Article
- Census of halide-binding sites in protein structures.Bioinformatics (Oxford, England) · 2020Article
- Structural diversity in the Mycobacteria DUF3349 superfamily.Protein science : a publication of the Protein Society · 2020Article
- Toward a structome of Acinetobacter baumannii drug targets.Protein science : a publication of the Protein Society · 2020Article
- Identification and Repurposing of Trisubstituted Harmine Derivatives as Novel Inhibitors ofMolecules (Basel, Switzerland) · 2020Article
- Exploring Structural Determinants of Inhibitor Affinity and Selectivity in Complexes with Histone Deacetylase 6.Journal of medicinal chemistry · 2020Article
- Targeting the Serine Pathway: A Promising Approach against Tuberculosis?Pharmaceuticals (Basel, Switzerland) · 2019Article
- Crystal structures of thiamine monophosphate kinase from Acinetobacter baumannii in complex with substrates and products.Scientific reports · 2019Article
- Cadmium SAD phasing at CuKα wavelength.F1000Research · 2019Article
- Ab initio structure solution of a proteolytic fragment using ARCIMBOLDO.Acta crystallographica. Section F, Structural biology communications · 2018Article
- Crystal structures of serum albumins from domesticated ruminants and their complexes with 3,5-diiodosalicylic acid.Acta crystallographica. Section D, Structural biology · 2017Article
- Structure of acid deoxyribonuclease.Nucleic acids research · 2017Article
- Fast iodide-SAD phasing for high-throughput membrane protein structure determination.Science advances · 2017Article
- Membrane protein structure determination by SAD, SIR, or SIRAS phasing in serial femtosecond crystallography using an iododetergent.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
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8 authors.
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Abstract
The Seattle Structural Genomics Center for Infectious Disease (SSGCID) focuses on the structure elucidation of potential drug targets from class A, B, and C infectious disease organisms. Many SSGCID targets are selected because they have homologs in other organisms that are validated drug targets with known structures. Thus, many SSGCID targets are expected to be solved by molecular replacement (MR), and reflective of this, all proteins are expressed in native form. However, many community request targets do not have homologs with known structures and not all internally selected targets readily solve by MR, necessitating experimental phase determination. We have adopted the use of iodide ion soaks and single wavelength anomalous dispersion (SAD) experiments as our primary method for de novo phasing. This method uses existing native crystals and in house data collection, resulting in rapid, low cost structure determination. Iodide ions are non-toxic and soluble at molar concentrations, facilitating binding at numerous hydrophobic or positively charged sites. We have used this technique across a wide range of crystallization conditions with successful structure determination in 16 of 17 cases within the first year of use (94% success rate). Here we present a general overview of this method as well as several examples including SAD phasing of proteins with novel folds and the combined use of SAD and MR for targets with weak MR solutions. These cases highlight the straightforward and powerful method of iodide ion SAD phasing in a high-throughput structural genomics environment.
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