ArticleClinical orthopaedics and related research2011
Engineered cartilage maturation regulates cytokine production and interleukin-1β response.
Article in Clinical orthopaedics and related research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
- Clinical Trial and In Vitro Study for the Role of Cartilage and Synovia in Acute Articular Infection.Mediators of inflammation · 2015Trial
- Phenotypic alterations in articulating joint cells: Role of mechanically loaded MSC secretome.Heliyon · 2025Article
- Use of bone marrow derived mesenchymal stem cells for the treatment of osteoarthritis: A retrospective long-term follow-up study.Journal of clinical orthopaedics and trauma · 2023Article
- Anti-inflammatory and pro-anabolic effects of 5-aminosalicylic acid on human inflammatory osteoarthritis models.Journal of orthopaedic translation · 2023Article
- Review
- Chondrocyte and mesenchymal stem cell derived engineered cartilage exhibits differential sensitivity to pro-inflammatory cytokines.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- Vascular Endothelial Growth Factor Sequestration Enhances In Vivo Cartilage Formation.International journal of molecular sciences · 2017Article
- Effects of sesamin on chondroitin sulfate proteoglycan synthesis induced by interleukin-1beta in human chondrocytes.BMC complementary and alternative medicine · 2017Article
- Notochordal cell conditioned medium (NCCM) regenerates end-stage human osteoarthritic articular chondrocytes and promotes a healthy phenotype.Arthritis research & therapy · 2016Article
- Dexamethasone Release from Within Engineered Cartilage as a Chondroprotective Strategy Against Interleukin-1α.Tissue engineering. Part A · 2016Article
- Article
- Beneficial effects of cerium oxide nanoparticles in development of chondrocyte-seeded hydrogel constructs and cellular response to interleukin insults.Tissue engineering. Part A · 2014Article
- Scaffold-based delivery of a clinically relevant anti-angiogenic drug promotes the formation of in vivo stable cartilage.Tissue engineering. Part A · 2013Article
- Muscle cell-derived factors inhibit inflammatory stimuli-induced damage in hMSC-derived chondrocytes.Osteoarthritis and cartilage · 2013Article
- Antioxidation of decellularized stem cell matrix promotes human synovium-derived stem cell-based chondrogenesis.Stem cells and development · 2013Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBecause the injured joint has an actively inflammatory environment, the survival and repair potential of cartilage grafts may be influenced by inflammatory processes. Understanding the interactions of those processes with the graft may lead to concepts for pharmacologic or surgical solutions allowing improved cartilage repair. QUESTIONS/PURPOSES: We asked whether the maturation level of cartilaginous tissues generated in vitro by expanded human articular chondrocytes (HACs) modulate (1) the spontaneous production of cytokines and (2) the response to interleukin (IL)-1β.
methodsTwelve pellets/donor prepared with monolayer-expanded HACs (n = 6 donors) were evaluated at six different culture times for mRNA expression (n = 72) and spontaneous baseline release of monocyte chemoattractant protein (MCP)-1, IL-8, and transforming growth factor (TGF)-β1 (n = 72). We cultured 24 pellets/donor from each of four donors for 1 or 14 days (defined as immature and mature, respectively) and exposed the pellets to IL-1β for 3 days. MCP-1, IL-8, TGF-β1, and metalloprotease (MMP)-1 and MMP-13 were quantified in pellets and culture supernatants.
resultsBy increasing culture time, the spontaneous release of IL-8 and MCP-1 decreased (12.0- and 5.5-fold, respectively), whereas that of TGF-β1 increased (5.4-fold). As compared with immature pellets, mature pellets responded to IL-1β by releasing lower amounts of MMP-1 (2.9-fold) and MMP-13 (1.7-fold) and increased levels of IL-8, MCP-1, and TGF-β1 (1.5-, 5.0-, and 7.5-fold, respectively). IL-8 and MCP-1 promptly returned to baseline on withdrawal of IL-1β.
conclusionsOur observations suggest more mature cartilaginous tissues are more resistant to IL-1β exposure and can activate chemokines required to initiate tissue repair processes. CLINICAL RELEVANCE: The implantation of more mature cartilaginous tissues might provide superior graft survival and improve/accelerate cartilage repair.
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