ArticlePlant molecular biology2011
Transgene excision in pollen using a codon optimized serine resolvase CinH-RS2 site-specific recombination system.
Article in Plant molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 55 citations in OpenAlex.
- Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Transgene Bioconfinement: Don't Flow There.Plants (Basel, Switzerland) · 2023Review
- Confirmation of 'Pollen- and Seed-Specific Gene Deletor' System Efficiency for Transgene Excision from TransgenicInternational journal of molecular sciences · 2023Article
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- Plasmids as Key Players inInternational journal of molecular sciences · 2022Review
- Overexpression of SHORT VEGETATIVE PHASE-LIKE (SVL) in Populus delays onset and reduces abundance of flowering in field-grown trees.Horticulture research · 2021Article
- Insights Into Genetic and Molecular Elements for Transgenic Crop Development.Frontiers in plant science · 2020Article
- Transgene Biocontainment Strategies for Molecular Farming.Frontiers in plant science · 2020Review
- Construction of Marker-Free Genetically Modified Maize Using a Heat-Inducible Auto-Excision Vector.Genes · 2019Article
- Small serine recombination systems ParA-MRS and CinH-RS2 perform precise excision of plastid DNA.Plant biotechnology journal · 2017Article
- Pollen-mediated gene flow from transgenic to non-transgenic switchgrass (Panicum virgatum L.) in the field.BMC biotechnology · 2017Article
- Field-grown miR156 transgenic switchgrass reproduction, yield, global gene expression analysis, and bioconfinement.Biotechnology for biofuels · 2017Article
- The superwoman1-cleistogamy2 mutant is a novel resource for gene containment in rice.Plant biotechnology journal · 2017Article
- Gene stacking by recombinases.Plant biotechnology journal · 2016Review
- Generation of Marker- and/or Backbone-Free Transgenic Wheat Plants viaFrontiers in plant science · 2016Article
- A bacterial gene codA encoding cytosine deaminase is an effective conditional negative selectable marker in Glycine max.Plant cell reports · 2015Article
- Review
- Transgene flow: facts, speculations and possible countermeasures.GM crops & food · 2014Review
- Advanced genetic tools for plant biotechnology.Nature reviews. Genetics · 2013Review
- Excision of a selectable marker gene in transgenic banana using a Cre/lox system controlled by an embryo specific promoter.Plant molecular biology · 2013Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgene escape, a major environmental and regulatory concern in transgenic crop cultivation, could be alleviated by removing transgenes from pollen, the most frequent vector for transgene flow. A transgene excision vector containing a codon optimized serine resolvase CinH recombinase (CinH) and its recognition sites RS2 were constructed and transformed into tobacco (Nicotiana tabacum cv. Xanthi). CinH recombinase recognized 119 bp of nucleic acid sequences, RS2, in pollen and excised the transgene flanked by the RS2 sites. In this system, the pollen-specific LAT52 promoter from tomato was employed to control the expression of CinH recombinase. Loss of expression of a green fluorescent protein (GFP) gene under the control of the LAT59 promoter from tomato was used as an indicator of transgene excision. Efficiency of transgene excision from pollen was determined by flow cytometry (FCM)-based pollen screening. While a transgenic event in the absence of CinH recombinase contained about 70% of GFP-synthesizing pollen, three single-copy transgene events contained less than 1% of GFP-synthesizing pollen based on 30,000 pollen grains analyzed per event. This suggests that CinH-RS2 recombination system could be effectively utilized for transgene biocontainment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.