ArticlePloS one2011
Oocyte-targeted deletion reveals that hsp90b1 is needed for the completion of first mitosis in mouse zygotes.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
- Trial
- Expanding roles of N-glycosylation in the endoplasmic reticulum.Trends in cell biology · 2026Review
- Heat shock protein Gp96 (Grp94) in malaria: functional insights at the host-parasite interface and therapeutic perspectives.Frontiers in cell and developmental biology · 2026Review
- Investigation of the Role of miR-1236-3p in Heat Tolerance of American Shad (International journal of molecular sciences · 2025Article
- [High-resolution Differential Proteomic Analysis of Mouse Secondary Oocytes and First Polar Bodies].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2025Article
- Integrated transcriptome and microRNA analysis reveals molecular responses to high-temperature stress in the liver of American shad (Alosa sapidissima).BMC genomics · 2024Article
- Screening of temperature-responsive signalling molecules during sex differentiation in Asian yellow pond turtle (Mauremys mutica).BMC genomics · 2024Article
- The Role of Heat Shock Proteins in the Pathogenesis of Polycystic Ovarian Syndrome: A Review of the Literature.International journal of molecular sciences · 2023Review
- Dual spindles assemble in bovine zygotes despite the presence of paternal centrosomes.The Journal of cell biology · 2021Article
- Homozygous Mutations in BTG4 Cause Zygotic Cleavage Failure and Female Infertility.American journal of human genetics · 2020Article
- Regulation of the Endoplasmic Reticulum Stress by BIP/GRP78 is involved in Meiotic Maturation of Porcine OocytesDevelopment & reproduction · 2017Article
- Relief of endoplasmic reticulum stress enhances DNA damage repair and improves development of pre-implantation embryos.PloS one · 2017Article
- No effect of exogenous melatonin on development of cryopreserved metaphase II oocytes in mouse.Journal of animal science and biotechnology · 2015Article
- Protein quality control in the endoplasmic reticulum.F1000prime reports · 2014Review
- The promoter of the oocyte-specific gene, Oog1, functions in both male and female meiotic germ cells in transgenic mice.PloS one · 2013Article
- GRP94: An HSP90-like protein specialized for protein folding and quality control in the endoplasmic reticulum.Biochimica et biophysica acta · 2012Review
- Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundHsp90b1 is an endoplasmic reticulum (ER) chaperone (also named Grp94, ERp99, gp96,Targ2, Tra-1, Tra1, Hspc4) (MGI:98817) contributing with Hspa5 (also named Grp78, BIP) (MGI:95835) to protein folding in ER compartment. Besides its high protein expression in mouse oocytes, little is known about Hsp90b1 during the transition from oocyte-to-embryo. Because the constitutive knockout of Hsp90b1 is responsible for peri-implantation embryonic lethality, it was not yet known whether Hsp90b1 is a functionally important maternal factor. METHODOLOGY/
findingsTo circumvent embryonic lethality, we established an oocyte-specific conditional knockout line taking advantage of the more recently created floxed Hsp90b1 line (Hsp90b1(flox), MGI:3700023) in combination with the transgenic mouse line expressing the cre recombinase under the control of zona pellucida 3 (ZP3) promoter (Zp3-cre, MGI:2176187). Altered expression of Hsp90b1 in growing oocytes provoked a limited, albeit significant reduction of the zona pellucida thickness but no obvious anomalies in follicular growth, meiotic maturation or fertilization. Interestingly, mutant zygotes obtained from oocytes lacking Hsp90b1 were unable to reach the 2-cell stage. They exhibited either a G2/M block or, more frequently an abnormal mitotic spindle leading to developmental arrest. Despite the fact that Hspa5 displayed a similar profile of expression as Hsp90b1, we found that HSPA5 and HSP90B1 did not fully colocalize in zygotes suggesting distinct function for the two chaperones. Consequently, even if HSPA5 was overexpressed in Hsp90b1 mutant embryos, it did not compensate for HSP90B1 deficiency. Finally, further characterization of ER compartment and cytoskeleton revealed a defective organization of the cytoplasmic region surrounding the mutant zygotic spindle.
conclusionsOur findings demonstrate that the maternal contribution of Hsp90b1 is critical for the development of murine zygotes. All together our data indicate that Hsp90b1 is involved in unique and specific aspects of the first mitosis, which brings together the maternal and paternal genomes on a single spindle.
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