ArticleMolecular and cellular biology2011
Elongator protein 3b negatively regulates ribosomal DNA transcription in african trypanosomes.
Article in Molecular and cellular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Article
- A systematic analysis ofGenome research · 2021Article
- A nuclear enterprise: zooming in on nuclear organization and gene expression control in the African trypanosome.Parasitology · 2021Review
- Spatial integration of transcription and splicing in a dedicated compartment sustains monogenic antigen expression in African trypanosomes.Nature microbiology · 2021Article
- Review
- Review
- Involvement in surface antigen expression by a moonlighting FG-repeat nucleoporin in trypanosomes.Molecular biology of the cell · 2018Article
- Epigenetic Regulation of Transcription in Trypanosomatid Protozoa.Current genomics · 2018Review
- Nucleolar Division in the Promastigote Stage ofBioMed research international · 2018Article
- GT-rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes.The EMBO journal · 2017Article
- Ribosomal RNA gene transcription in trypanosomes.Parasitology research · 2014Review
- Trypanosoma cruzi bromodomain factor 3 binds acetylated α-tubulin and concentrates in the flagellum during metacyclogenesis.Eukaryotic cell · 2014Article
- Antigenic variation in African trypanosomes: the importance of chromosomal and nuclear context in VSG expression control.Cellular microbiology · 2013Review
- TDP1 is an HMG chromatin protein facilitating RNA polymerase I transcription in African trypanosomes.Nucleic acids research · 2013Article
- Cell-cycle-regulated control of VSG expression site silencing by histones and histone chaperones ASF1A and CAF-1b in Trypanosoma brucei.Nucleic acids research · 2012Article
- Review
- Lysine acetylation: elucidating the components of an emerging global signaling pathway in trypanosomes.Journal of biomedicine & biotechnology · 2012Review
- Regulation of ribosomal RNA production by RNA polymerase I: does elongation come first?Genetics research international · 2012Article
Corrections and comments
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Authors and funding
2 authors.
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Abstract
Eukaryotic cells limit ribosomal DNA (rDNA) transcription by RNA polymerase I (RNAP-I) to maintain genome integrity. African trypanosomes present an excellent model for studies on RNAP-I regulation because they possess a bifunctional RNAP-I and because RNAP-II transcription appears unregulated. Since Elp3, the catalytic component of Elongator, controls RNAP-II transcription in yeast and human cells, we predicted a role for a trypanosome Elp3-related protein, ELP3a or ELP3b, in RNAP-I regulation. elp3b null and conditional strains specifically exhibited resistance to a transcription elongation inhibitor, suggesting that ELP3b negatively impacts elongation. Nascent RNA analysis and expression of integrated reporter cassettes supported this interpretation and revealed negative control of rDNA transcription. ELP3b specifically localized to the nucleolus, and ELP3b loss rendered cells hypersensitive to DNA damage and to translation inhibition, suggesting that anti-Elongator function was important to maintain genome integrity rather than to modulate ribosome production. Finally, ELP3b displayed discrimination between RNAP-I compartments in the same cell. Our results establish ELP3b as a major negative regulator of rDNA transcription and extend the roles of the Elp3-related proteins to RNAP-I transcription units. ELP3b is also the first trypanosome protein shown to distinguish between rDNA and variant surface glycoprotein transcription within different RNAP-I compartments.
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