ArticleJournal of bacteriology2011
Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- RfaH May Oppose Silencing by H-NS and YmoA Proteins during Transcription Elongation.Journal of bacteriology · 2022Article
- Xenogeneic silencing strategies in bacteria are dictated by RNA polymerase promiscuity.Nature communications · 2022Article
- Mastering the control of the Rho transcription factor for biotechnological applications.Applied microbiology and biotechnology · 2021Review
- A How-To Guide for Mode of Action Analysis of Antimicrobial Peptides.Frontiers in cellular and infection microbiology · 2020Review
- The Rho-Dependent Transcription Termination Is Involved in Broad-Spectrum Antibiotic Susceptibility inFrontiers in microbiology · 2020Article
- Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli.Nucleic acids research · 2018Article
- A Chemical-Genomic Screen of Neglected Antibiotics Reveals Illicit Transport of Kasugamycin and Blasticidin S.PLoS genetics · 2016Article
- Regulation of transcription elongation and termination.Biomolecules · 2015Review
- Article
- Regulation of Transcript Elongation.Annual review of microbiology · 2015Review
- Lethal synergy involving bicyclomycin: an approach for reviving old antibiotics.The Journal of antimicrobial chemotherapy · 2014Article
- Involvement of stress-related genes polB and PA14_46880 in biofilm formation of Pseudomonas aeruginosa.Infection and immunity · 2014Article
- Design and development of synthetic microbial platform cells for bioenergy.Frontiers in microbiology · 2013Article
- Rho and NusG suppress pervasive antisense transcription in Escherichia coli.Genes & development · 2012Article
- Modulation of Rho-dependent transcription termination in Escherichia coli by the H-NS family of proteins.Journal of bacteriology · 2011Article
- The Sm-like RNA chaperone Hfq mediates transcription antitermination at Rho-dependent terminators.The EMBO journal · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have screened the entire KEIO collection of 3,985 single-gene knockouts in Escherichia coli for increased susceptibility or resistance to the antibiotic bicyclomycin (BCM), a potent inhibitor of the transcription termination factor Rho. We also compared the results to those of a recent study we conducted with a large set of antibiotics (A. Liu et al., Antimicrob. Agents Chemother. 54:1393-1403, 2010). We find that deletions of many different types of genes increase sensitivity to BCM. Some of these are involved in multidrug sensitivity/resistance, whereas others are specific for BCM. Mutations in a number of DNA recombination and repair genes increase BCM sensitivity, indicating that DNA damage leading to single- and double-strand breaks is a downstream effect of Rho inhibition. MDS42, which is deleted for all cryptic prophages and insertion elements (G. Posfai et al., Science 312:1044-1046, 2006), or W3102 deleted for the rac prophage-encoded kil gene, are partially resistant to BCM (C. J. Cardinale et al., Science 230:935-938, 2008). Deletion of cryptic prophages also overcomes the increased BCM sensitivity in some but not all mutants examined here. Deletion of the hns gene renders the cell more sensitive to BCM even in the Δkil or MDS42 background. This suggests that BCM activates additional modes of cell death independent of Kil and that these could provide a target to potentiate BCM killing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.